J Korean Neuropsychiatr Assoc.  2000 Sep;39(5):920-927.

Neurotrophins and IGFs Attenuated Haloperidol-Induced Neuronal Apoptosis in Mouse Cortical Cell Cultures

• Kang HJ
• Yoon KJ
• Gwag BJ
• Chung YK
• Noh JS

Abstract

OBJECTIVES
We examined the effects of neurotrophins and insulin-like growth factors on cell death induced by haloperidol, a typical anti-psychotic agent. METHOD: Neocortices from 14- or 15-daysold fetal mice for neuron-glia co-cultures were used for this experiment. RESULT: Twenty-four hours treatment of mouse cortical cell cultures with 30 M haloperidol-induced wide spread neuronal apoptosis characterized by cell body shrinkage, DNA fragmentation and condensation. Concurrent treatment with growth factors, BDNF, NT4/5, IGF-I and IGF-II, protect the neurons from the haloperidol-induced neuronal apoptosis(HINA) in a dose dependent manner(10-100ng/ml).
CONCLUSION
The present study suggests the possibility that haloperidol toxicity can be hampered with growth factors. Further study about the mechanism underlying the protective capacity of the growth factors on HINA may lead to the development of the new protective strategy for tardive dyskinesia.

Keyword

Haloperidol; Apoptosis; Neuronal death; BDNF; NT4/5; IGF-I; IGF-II

MeSH Terms

Animals
Apoptosis*
Brain-Derived Neurotrophic Factor
Cell Culture Techniques*
Cell Death
Coculture Techniques
DNA Fragmentation
Haloperidol
Insulin-Like Growth Factor I
Insulin-Like Growth Factor II
Intercellular Signaling Peptides and Proteins
Mice*
Movement Disorders
Neocortex
Nerve Growth Factors*
Neurons*
Somatomedins
Brain-Derived Neurotrophic Factor
Haloperidol
Insulin-Like Growth Factor I
Insulin-Like Growth Factor II
Intercellular Signaling Peptides and Proteins
Nerve Growth Factors
Somatomedins