Restor Dent Endod.  2014 May;39(2):109-114.

Antibacterial properties of composite resins incorporating silver and zinc oxide nanoparticles on Streptococcus mutans and Lactobacillus

• Kasraei S
• Sami L
• Hendi S
• AliKhani M
• Rezaei-Soufi L
• Khamverdi

Abstract

OBJECTIVES
Recurrent caries was partly ascribed to lack of antibacterial properties in composite resin. Silver and zinc nanoparticles are considered to be broad-spectrum antibacterial agents. The aim of the present study was to evaluate the antibacterial properties of composite resins containing 1% silver and zinc-oxide nanoparticles on Streptococcus mutans and Lactobacillus.
MATERIALS AND METHODS
Ninety discoid tablets containing 0%, 1% nano-silver and 1% nano zinc-oxide particles were prepared from flowable composite resin (n = 30). The antibacterial properties of composite resin discs were evaluated by direct contact test. Diluted solutions of Streptococcus mutans (PTCC 1683) and Lactobacillus (PTCC 1643) were prepared. 0.01 mL of each bacterial species was separately placed on the discs. The discs were transferred to liquid culture media and were incubated at 37degrees C for 8 hr. 0.01 mL of each solution was cultured on blood agar and the colonies were counted. Data was analyzed with Kruskall-Wallis and Mann-Whitney U tests.
RESULTS
Composites containing nano zinc-oxide particles or silver nanoparticles exhibited higher antibacterial activity against Streptococcus mutans and Lactobacillus compared to the control group (p < 0.05). The effect of zinc-oxide on Streptococcus mutans was significantly higher than that of silver (p < 0.05). There were no significant differences in the antibacterial activity against Lactobacillus between composites containing silver nanoparticles and those containing zinc-oxide nanoparticles.
CONCLUSIONS
Composite resins containing silver or zinc-oxide nanoparticles exhibited antibacterial activity against Streptococcus mutans and Lactobacillus.

Keyword

Anti-bacterial agents; Composite resin; Nanoparticles; Silver; Zinc oxide

MeSH Terms

Agar
Anti-Bacterial Agents
Composite Resins*
Culture Media
Lactobacillus*
Nanoparticles*
Silver*
Streptococcus mutans*
Streptococcus*
Tablets
Zinc Oxide*
Zinc*
Agar
Anti-Bacterial Agents
Composite Resins
Culture Media
Silver
Tablets
Zinc
Zinc Oxide

Figure

• 

  Figure 1 SEM-EDX image of the surface of the composite resin samples containing 1% of silver and zinc-oxide nanoparticles.

Reference

1. Opdam NJ, Bronkhorst EM, Roeters JM, Loomans BA. A retrospective clinical study on longevity of posterior composite and amalgam restorations. Dent Mater. 2007; 23:2–8.

2. Sadowsky SJ. An overview of treatment considerations for esthetic restorations: a review of the literature. J Prosthet Dent. 2006; 96:433–442.

3. Pereira-Cenci T, Cenci MS, Fedorowicz Z, Marchesan MA. Antibacterial agents in composite restorations for the prevention of dental caries. Cochrane Database Syst Rev. 2009; (3):CD007819.

4. Papagiannoulis L, Kakaboura A, Eliades G. In vivo vs in vitro anticariogenic behavior of glass-ionomer and resin composite restorative materials. Dent Mater. 2002; 18:561–569.

5. Thomé T, Mayer MP, Imazato S, Geraldo-Martins VR, Marques MM. In vitro analysis of inhibitory effects of the antibacterial monomer MDPB-containing restorations on the progression of secondary root caries. J Dent. 2009; 37:705–711.

6. Kim S, Song M, Roh BD, Park SH, Park JW. Inhibition of Streptococcus mutans biofilm formation on composite resins containing ursolic acid. Restor Dent Endod. 2013; 38:65–72.

7. Kim JS, Shin DH. Inhibitory effect on Streptococcus mutans and mechanical properties of the chitosan containing composite resin. Restor Dent Endod. 2013; 38:36–42.

8. Hernández-Sierra JF, Ruiz F, Pena DC, Martínez-Gutiérrez F, Martínez AE, Guillén Ade J, Tapia-Pérez H, Castañón GM. The antimicrobial sensitivity of Streptococcus mutans to nanoparticles of silver, zinc oxide, and gold. Nanomedicine. 2008; 4:237–240.

9. Bürgers R, Eidt A, Frankenberger R, Rosentritt M, Schweikl H, Handel G, Hahnel S. The anti-adherence activity and bactericidal effect of microparticulate silver additives in composite resin materials. Arch Oral Biol. 2009; 54:595–601.

10. Melo MA, Cheng L, Zhang K, Weir MD, Rodrigues LK, Xu HH. Novel dental adhesives containing nanoparticles of silver and amorphous calcium phosphate. Dent Mater. 2013; 29:199–210.

11. Tavassoli Hojati S, Alaghemand H, Hamze F, Ahmadian Babaki F, Rajab-Nia R, Rezvani MB, Kaviani M, Atai M. Antibacterial, physical and mechanical properties of flowable resin composites containing zinc oxide nanoparticles. Dent Mater. 2013; 29:495–505.

12. Xu T, Xie CS. Tetrapod-like nano-particle ZnO/acrylic resin composite and its multi-function property. Prog Org Coat. 2003; 46:297–301.

13. Li LH, Deng JC, Deng HR, Liu ZL, Li XL. Preparation, characterization and antimicrobial activities of chitosan/Ag/ZnO blend films. Chem Eng J. 2010; 160:378–382.

14. Sheng J, Nguyen PT, Marquis RE. Multi-target antimicrobial actions of zinc against oral anaerobes. Arch Oral Biol. 2005; 50:747–757.

15. Cummins D. Zinc citrate/Triclosan: a new anti-plaque system for the control of plaque and the prevention of gingivitis: short-term clinical and mode of action studies. J Clin Periodontol. 1991; 18:455–461.

16. Pinto RJ, Marques PA, Neto CP, Trindade T, Daina S, Sadocco P. Antibacterial activity of nanocomposites of silver and bacterial or vegetable cellulosic fibers. Acta Biomater. 2009; 5:2279–2289.

17. Martinez-Gutierrez F, Olive PL, Banuelos A, Orrantia E, Nino N, Sanchez EM, Ruiz F, Bach H, Av-Gay Y. Synthesis, characterization, and evaluation of antimicrobial and cytotoxic effect of silver and titanium nanoparticles. Nanomedicine. 2010; 6:681–688.

18. Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci. 2004; 275:177–182.

19. Kim JS, Kuk E, Yu KN, Kim JH, Park SJ, Lee HJ, Kim SH, Park YK, Park YH, Hwang CY, Kim YK, Lee YS, Jeong DH, Cho MH. Antimicrobial effects of silver nanoparticles. Nanomedicine. 2007; 3:95–101.

20. Spacciapoli P, Buxton D, Rothstein D, Friden P. Antimicrobial activity of silver nitrate against periodontal pathogens. J Periodontal Res. 2001; 36:108–113.

21. Sahoo SK, Parveen S, Panda JJ. The present and future of nanotechnology in human health care. Nanomedicine. 2007; 3:20–31.

22. Rai M, Yadav A, Gade A. Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv. 2009; 27:76–83.

23. Li LH, Deng JC, Deng HR, Liu ZL, Xin L. Synthesis and characterization of chitosan/ZnO nanoparticle composite membranes. Carbohydr Res. 2010; 345:994–998.

24. Kumar R, Münstedt H. Silver ion release from antimicrobial polyamide/silver composites. Biomaterials. 2005; 26:2081–2088.

25. Svanberg M, Mjör IA, Orstavik D. Mutans streptococci in plaque from margins of amalgam, composite, and glass-ionomer restorations. J Dent Res. 1990; 69:861–864.

26. Bundy KJ, Butler MF, Hochman RF. An investigation of the bacteriostatic properties of pure metals. J Biomed Mater Res. 1980; 14:653–663.

27. Applerot G, Perkas N, Amirian G, Girshevitz O, Gedanken A. Coating of glass with ZnO via ultrasonic irradiation and a study of its antibacterial properties. Appl Surf Sci. 2009; 256:S3–S8.

28. Imazato S. Antibacterial properties of resin composites and dentin bonding systems. Dent Mater. 2003; 19:449–457.

29. Aydin Sevinç B, Hanley L. Antibacterial activity of dental composites containing zinc oxide nanoparticles. J Biomed Mater Res B Appl Biomater. 2010; 94:22–31.

30. Beyth N, Houri-Haddad Y, Baraness-Hadar L, Yudovin-Farber I, Domb AJ, Weiss EI. Surface antimicrobial activity and biocompatibility of incorporated polyethylenimine nanoparticles. Biomaterials. 2008; 29:4157–4163.

31. Yoshida K, Tanagawa M, Matsumoto S, Yamada T, Atsuta M. Antibacterial activity of resin composites with silver-containing materials. Eur J Oral Sci. 1999; 107:290–296.

32. Spencer CG, Campbell PM, Buschang PH, Cai J, Honeyman AL. Antimicrobial effects of zinc oxide in an orthodontic bonding agent. Angle Orthod. 2009; 79:317–322.

33. Tanagawa M, Yoshida K, Matsumoto S, Yamada T, Atsuta M. Inhibitory effect of antibacterial resin composite against Streptococcus mutans. Caries Res. 1999; 33:366–371.

34. Pandit S, Kim GR, Lee MH, Jeon JG. Evaluation of Streptococcus mutans biofilms formed on fluoride releasing and non fluoride releasing resin composites. J Dent. 2011; 39:780–787.

35. van Dijken JW, Kalfas S, Litra V, Oliveby A. Fluoride and mutans streptococci levels in plaque on aged restorations of resin-modified glass ionomer cement, compomer and resin composite. Caries Res. 1997; 31:379–383.

36. Wiegand A, Buchalla W, Attin T. Review on fluoride-releasing restorative materials-fluoride release and uptake characteristics, antibacterial activity and influence on caries formation. Dent Mater. 2007; 23:343–362.

37. Schmidlin PR, Schätzle M, Fischer J, Attin T. Bonding of brackets using a caries-protective adhesive patch. J Dent. 2008; 36:125–129.