Restor Dent Endod.  2017 Feb;42(1):39-47. 10.5395/rde.2017.42.1.39.

In vitro study of Streptococcus mutans adhesion on composite resin coated with three surface sealants

Affiliations
  • 1Department of Medical and Biological Engineering, Graduate School, Daegu, Korea.
  • 2Department of Dental Biomaterials, School of Dentistry, Daegu, Korea. tykwon@knu.ac.kr
  • 3Institute for Biomaterials Research and Development, Kyungpook National University, Daegu, Korea.

Abstract


OBJECTIVES
Although the coating of surface sealants to dental composite resin may potentially reduce bacterial adhesion, there seems to be little information regarding this issue. This preliminary in vitro study investigated the adhesion of Streptococcus mutans (S. mutans) on the dental composite resins coated with three commercial surface sealants.
MATERIALS AND METHODS
Composite resin (Filtek Z250) discs (8 mm in diameter, 1 mm in thickness) were fabricated in a mold covered with a Mylar strip (control). In group PoGo, the surfaces were polished with PoGo. In groups PS, OG, and FP, the surfaces polished with PoGo were coated with the corresponding surface sealants (PermaSeal, PS; OptiGuard, OG; Fortify Plus, FP). The surfaces of the materials and S. mutans cells were characterized by various methods. S. mutans adhesion to the surfaces was quantitatively evaluated using flow cytometry (n = 9).
RESULTS
Group OG achieved the lowest water contact angle among all groups tested (p < 0.001). The cell surface of S. mutans tested showed hydrophobic characteristics. Group PoGo exhibited the greatest bacterial adhesion among all groups tested (p < 0.001). The sealant-coated groups showed statistically similar (groups PS and FP, p > 0.05) or significantly lower (group OG, p < 0.001) bacterial adhesion when compared with the control group.
CONCLUSIONS
The application of the surface sealants significantly reduced S. mutans adhesion to the composite resin polished with the PoGo.

Keyword

Composite resins; Streptococcus mutans; Surface properties

MeSH Terms

Bacterial Adhesion
Composite Resins
Flow Cytometry
Fungi
In Vitro Techniques*
Streptococcus mutans*
Streptococcus*
Surface Properties
Water
Composite Resins
Water

Figure

  • Figure 1 Representative scanning electron microscopy images of the composite resin surfaces finished with Mylar (a), polished with PoGo (b), and coated with PermaSeal (c), OptiGuard (d), and Fortify Plus (e), respectively (original magnification ×5,000, bar = 2 µm).

  • Figure 2 Affinity of S. mutans towards the test hydrocarbons (closed circles, hexadecane; open triangles, toluene) as a function of hydrocarbon volume. Aqueous bacterial suspensions were mixed with varying volumes of hydrocarbon. Results are expressed as a percentage of the initial absorbance of the aqueous suspension as a function of hydrocarbon volume. The photo inside the graph shows adherence of S. mutans to hexadecane as a function of increasing hexadecane volume. From left to right: 0, 0.025, 0.05, 0.1, 0.2, 0.3, and 0.4 mL hexadecane added. When toluene was substituted for hexadecane, a similar trend was observed. S. mutans, Streptococcus mutans.

  • Figure 3 Quantification of S. mutans adhesion for each group using flow cytometry. (a) Flow cytometry dot plots; (b) Bacterial counts per unit area for each group (n = 9) (green, live bacteria; red, dead bacteria). The vertical bar indicates standard deviation. Mean values with different superscripts (a, b, and c) are significantly different (p < 0.05). Means were log10 transformed prior to analysis. L, live bacteria; D, dead bacteria; B, reference microsphere beads; S. mutans, Streptococcus mutans.

  • Figure 4 Representative fluorescence microscope images of S. mutans adhered on the resin specimens. SYTO 9-labeled live bacteria images (upper), propydium iodide-labeled dead bacteria images (central), and merged images of LIVE/DEAD bacteria (lower) (original magnification ×100, bar = 200 µm). S. mutans, Streptococcus mutans; PI, propydium iodide; PS, PermaSeal (Ultradent Products Inc.); OG, OptiGuard (Kerr Corp.); FP, Fortify Plus (Bisco Inc.).


Reference

1. Bollen CM, Lambrechts P, Quirynen M. Comparison of surface roughness of oral hard materials to the threshold surface roughness for bacterial plaque retention: a review of the literature. Dent Mater. 1997; 13:258–269.
Article
2. Cilli R, de Mattos MC, Honorio HM, Rios D, de Araujo PA, Prakki A. The role of surface sealants in the roughness of composites after a simulated toothbrushing test. J Dent. 2009; 37:970–977.
Article
3. Bürgers R, Cariaga T, Müller R, Rosentritt M, Reischl U, Handel G, Hahnel S. Effects of aging on surface properties and adhesion of Streptococcus mutans on various fissure sealants. Clin Oral Investig. 2009; 13:419–426.
Article
4. Dickinson GL, Leinfelder KF, Mazer RB, Russell CM. Effect of surface penetrating sealant on wear rate of posterior composite resins. J Am Dent Assoc. 1990; 121:251–255.
Article
5. dos Santos PH, Consani S, Correr Sobrinho L, Coelho Sinhoreti MA. Effect of surface penetrating sealant on roughness of posterior composite resins. Am J Dent. 2003; 16:197–201.
6. Prakki A, Ribeiro IW, Cilli R, Mondelli RF. Assessing the tooth-restoration interface wear resistance of two cementation techniques: effect of a surface sealant. Oper Dent. 2005; 30:739–746.
7. Ramos RP, Chimello DT, Chinelatti MA, Dibb RG, Mondelli J. Effect of three surface sealants on marginal sealing of Class V composite resin restorations. Oper Dent. 2000; 25:448–453.
8. dos Santos PH, Pavan S, Suzuki TY, Briso AL, Assunção WG, Sinhoreti MA, Correr-Sobrinho L, Consani S. Effect of fluid resins on the surface roughness and topography of resin composite restorations analyzed by atomic force microscope. J Mech Behav Biomed Mater. 2011; 4:433–439.
Article
9. An YH, Friedman RJ. Concise review of mechanisms of bacterial adhesion to biomaterial surfaces. J Biomed Mater Res. 1998; 43:338–348.
Article
10. Türkün LS, Türkün M. The effect of one-step polishing system on the surface roughness of three esthetic resin composite materials. Oper Dent. 2004; 29:203–211.
11. Martin MA, Pfaller MA, Massanari RM, Wenzel RP. Use of cellular hydrophobicity, slime production, and species identification markers for the clinical significance of coagulase-negative staphylococcal isolates. Am J Infect Control. 1989; 17:130–135.
Article
12. Nostro A, Cannatelli MA, Crisafi G, Musolino AD, Procopio F, Alonzo V. Modifications of hydrophobicity, in vitro adherence and cellular aggregation of Streptococcus mutans by Helichrysum italicum extract. Lett Appl Microbiol. 2004; 38:423–427.
Article
13. Zhou L, Tong Z, Wu G, Feng Z, Bai S, Dong Y, Ni L, Zhao Y. Parylene coating hinders Candida albicans adhesion to silicone elastomers and denture bases resin. Arch Oral Biol. 2010; 55:401–409.
Article
14. Montanaro L, Campoccia D, Rizzi S, Donati ME, Breschi L, Prati C, Arciola CR. Evaluation of bacterial adhesion of Streptococcus mutans on dental restorative materials. Biomaterials. 2004; 25:4457–4463.
Article
15. Kang SH, Lee HJ, Hong SH, Kim KH, Kwon TY. Influence of surface characteristics on the adhesion of Candida albicans to various denture lining materials. Acta Odontol Scand. 2013; 71:241–248.
Article
16. Orth R, O'Brien-Simpson N, Dashper S, Walsh K, Reynolds E. An efficient method for enumerating oral spirochetes using flow cytometry. J Microbiol Methods. 2010; 80:123–128.
Article
17. Pan H, Feng J, Cerniglia CE, Chen H. Effects of Orange II and Sudan III azo dyes and their metabolites on Staphylococcus aureus. J Ind Microbiol Biotechnol. 2011; 38:1729–1738.
Article
18. Li F, Chen J, Chai Z, Zhang L, Xiao Y, Fang M, Ma S. Effects of a dental adhesive incorporating antibacterial monomer on the growth, adherence and membrane integrity of Streptococcus mutans. J Dent. 2009; 37:289–296.
Article
19. Quirynen M, Bollen CM. The influence of surface roughness and surface-free energy on supra- and subgingival plaque formation in man. A review of the literature. J Clin Periodontol. 1995; 22:1–14.
Article
20. Burdz TV, Wolfe J, Kabani A. Evaluation of sputum decontamination methods for Mycobacterium tuberculosis using viable colony counts and flow cytometry. Diagn Microbiol Infect Dis. 2003; 47:503–509.
Article
21. Gunasekera TS, Attfield PV, Veal DA. A flow cytometry method for rapid detection and enumeration of total bacteria in milk. Appl Environ Microbiol. 2000; 66:1228–1232.
Article
22. Filoche SK, Coleman MJ, Angker L, Sissons CH. A fluorescence assay to determine the viable biomass of microcosm dental plaque biofilms. J Microbiol Methods. 2007; 69:489–496.
Article
23. Cai Y, Strømme M, Welch K. Bacteria viability assessment after photocatalytic treatment. 3 Biotech. 2014; 4:149–157.
Article
24. Antonucci JM, Fowler BO, Dickens SH, Richards ND. Novel dental resins from trialkoxysilanes and dental monomers by in situ formation of oligomeric silyl ethers and silsesquioxanes. Polym Prepr. 2002; 43:633–634.
25. Pereira SG, Osorio R, Toledano M, Nunes TG. Evaluation of two Bis-GMA analogues as potential monomer diluents to improve the mechanical properties of light-cured composite resins. Dent Mater. 2005; 21:823–830.
Article
26. Tay FR, Pashley DH, Kapur RR, Carrilho MR, Hur YB, Garrett LV, Tay KC. Bonding BisGMA to dentin-a proof of concept for hydrophobic dentin bonding. J Dent Res. 2007; 86:1034–1039.
Article
27. Wilson F, Heath JR, Watts DC. Finishing composite restorative materials. J Oral Rehabil. 1990; 17:79–87.
Article
28. Ozel E, Korkmaz Y, Attar N, Karabulut E. Effect of one-step polishing systems on surface roughness of different flowable restorative materials. Dent Mater J. 2008; 27:755–764.
Article
29. Korkmaz Y, Ozel E, Attar N, Aksoy G. The influence of one-step polishing systems on the surface roughness and microhardness of nanocomposites. Oper Dent. 2008; 33:44–50.
Article
30. Kim MJ, Kim YK, Kim KH, Kwon TY. Shear bond strengths of various luting cements to zirconia ceramic: surface chemical aspects. J Dent. 2011; 39:795–803.
Article
31. Hay KM, Dragila MI, Liburdy J. Theoretical model for the wetting of a rough surface. J Colloid Interface Sci. 2008; 325:472–477.
Article
32. Bollen CM, Papaioanno W, Van Eldere J, Schepers E, Quirynen M, van Steenberghe D. The influence of abutment surface roughness on plaque accumulation and peri-implant mucositis. Clin Oral Implants Res. 1996; 7:201–211.
Article
33. Claro-Pereira D, Sampaio-Maia B, Ferreira C, Rodrigues A, Melo LF, Vasconcelos MR. In situ evaluation of a new silorane-based composite resin's bioadhesion properties. Dent Mater. 2011; 27:1238–1245.
Article
34. Kawai K, Leinfelder KF. Effect of surface-penetrating sealant on composite wear. Dent Mater. 1993; 9:108–113.
Article
35. Busscher HJ, van Pelt AWJ, de Boer P, de Jong HP, Arends J. The effect of surface roughening of polymers on measured contact angles of liquids. Colloids Surf. 1984; 9:319–331.
Article
36. Kim YK, Son JS, Kim KH, Kwon TY. Influence of surface energy parameters of dental self-adhesive resin cements on bond strength to dentin. J Adhes Sci Technol. 2013; 27:1778–1789.
Article
37. Buergers R, Schneider-Brachert W, Hahnel S, Rosentritt M, Handel G. Streptococcal adhesion to novel low-shrink silorane-based restorative. Dent Mater. 2009; 25:269–275.
Article
38. Rüttermann S, Bergmann N, Beikler T, Raab WH, Janda R. Bacterial viability on surface-modified resin-based dental restorative materials. Arch Oral Biol. 2012; 57:1512–1521.
Article
39. Mei L, Busscher HJ, van der Mei HC, Chen Y, de Vries J, Ren Y. Oral bacterial adhesion forces to biomaterial surfaces constituting the bracket-adhesive-enamel junction in orthodontic treatment. Eur J Oral Sci. 2009; 117:419–426.
Article
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