Lab Anim Res.  2015 Dec;31(4):198-203. 10.5625/lar.2015.31.4.198.

Curcumin attenuates the middle cerebral artery occlusion-induced reduction in gamma-enolase expression in an animal model

Affiliations
  • 1Department of Anatomy, College of Veterinary Medicine, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Korea. pokoh@gnu.ac.kr

Abstract

Curcumin exerts a protective effect in cerebral ischemia through its anti-oxidant and anti-inflammatory activities. gamma-enolase is a glycolytic enzyme expressed in neurons that is known to exerts a neuroprotective effect. We investigated whether curcumin regulates gamma-enolase expression in focal cerebral ischemic injury in rats. Middle cerebral artery occlusion (MCAO) was performed to induce focal cerebral ischemia. Adult male rats were injected intraperitoneally with either vehicle or curcumin (50 mg/kg) 1 h after MCAO and cerebral cortex tissues were isolated 24 h after MCAO. We found that MCAO-induced injury resulted in a reduction in gamma-enolase expression in vehicle-treated animals using a proteomics approach. However, this reduction was attenuated in animals with MCAO treated with curcumin. Reverse-transcription PCR and Western blot analyses also showed that curcumin treatment prevented the MCAO injury-induced reduction in gamma-enolase expression. The results of this study suggest that curcumin exerts its neuroprotective function in focal cerebral ischemia by regulating the expression of gamma-enolase.

Keyword

gamma-enolase; curcumin; neuroprotection

MeSH Terms

Adult
Animals*
Blotting, Western
Brain Ischemia
Cerebral Cortex
Curcumin*
Humans
Infarction, Middle Cerebral Artery
Male
Middle Cerebral Artery*
Models, Animal*
Neurons
Neuroprotective Agents
Phosphopyruvate Hydratase*
Polymerase Chain Reaction
Proteomics
Rats
Curcumin
Neuroprotective Agents
Phosphopyruvate Hydratase
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