J Korean Acad Conserv Dent.  2010 May;35(3):152-163. 10.5395/JKACD.2010.35.3.152.

Gene expression profiling in human dental pulp cells treated with mineral trioxide aggregate

• Kim YB
• Shon WJ
• Lee W
• Kum KY
• Baek SH
• Bae KS

• KMID: 2294341
• DOI: http://doi.org/10.5395/JKACD.2010.35.3.152

Abstract

This study investigated the changes in gene expression when mineral trioxide aggregate (MTA) was applied in vitro to human dental pulp cells (HDPCs). MTA in a teflon tube (diameter 10 mm, height 2 mm) was applied to HDPCs. Empty tube-applied HDPCs were used as negative control. For microarray analysis, total RNA was extracted at 6, 24, and 72 hrs after MTA application. The results were confirmed selectively by performing reverse transcriptase polymerase chain reaction for genes that showed changes of more than two-fold or less than half. Of the 24,546 genes, 109 genes were up-regulated greater than two-fold (e.g., FOSB, THBS1, BHLHB2, EDN1, IL11, FN1, COL10A1, and TUFT1) and 69 genes were down-regulated below 50% (e.g., SMAD6 and DCN). These results suggest that MTA, rather than being a bio-inert material, may have potential to affect the proliferation and differentiation of pulp cells in various ways.

Keyword

Microarray; Mineral trioxide aggregate; Dental pulp capping; Human dental pulp cell; Differentiation; Proliferation

MeSH Terms

Aluminum Compounds
Calcium Compounds
Dental Pulp
Dental Pulp Capping
Drug Combinations
Gene Expression
Gene Expression Profiling
Glutamates
Guanine
Humans
Interleukin-11
Microarray Analysis
Oxides
Polytetrafluoroethylene
Reverse Transcriptase Polymerase Chain Reaction
RNA
Silicates
Pemetrexed
Aluminum Compounds
Calcium Compounds
Drug Combinations
Glutamates
Guanine
Interleukin-11
Oxides
Polytetrafluoroethylene
RNA
Silicates

Figure

• 

  Figure 1 Differentially expressed genes (178 genes) up- or down-regulated by at least two-fold following a cellular treatment with MTA at every time point. (a) The biological processes of up-regulated 109 genes, (b) The biological processes of down-regulated 69 genes.

• 

  Figure 2 Optical density of messenger RNA measured by densitometry.

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