J Korean Acad Conserv Dent.  2011 Sep;36(5):397-408. 10.5395/JKACD.2011.36.5.397.

Comparison of gene expression profiles of human dental pulp cells treated with mineral trioxide aggregate and calcium hydroxide

• Kim YB
• Shon WJ
• Lee W
• Kum KY
• Baek SH
• Bae KS

• KMID: 2176577
• DOI: http://doi.org/10.5395/JKACD.2011.36.5.397

Abstract

OBJECTIVES
This study investigated changes in gene expressions concerning of differentiation, proliferation, mineralization and inflammation using Human-8 expression bead arrays when white Mineral Trioxide Aggregate and calcium hydroxide-containing cement were applied in vitro to human dental pulp cells (HDPCs).
MATERIALS AND METHODS
wMTA (white ProRoot MTA, Dentsply) and Dycal (Dentsply Caulk) in a Teflon tube (inner diameter 10 mm, height 1 mm) were applied to HDPCs. Empty tube-applied HDPCs were used as negative control. Total RNA was extracted at 3, 6, 9 and 24 hr after wMTA and Dycal application. The results of microarray were confirmed by reverse transcriptase polymerase chain reaction.
RESULTS
Out of the 24,546 genes, 43 genes (e.g., BMP2, FOSB, THBS1, EDN1, IL11, COL10A1, TUFT1, HMOX1) were up-regulated greater than two-fold and 25 genes (e.g., SMAD6, TIMP2, DCN, SOCS2, CEBPD, KIAA1199) were down-regulated below 50% by wMTA. Two hundred thirty nine genes (e.g., BMP2, BMP6, SMAD6, IL11, FOS, VEGFA, PlGF, HMOX1, SOCS2, CEBPD, KIAA1199) were up-regulated greater than two-fold and 358 genes (e.g., EDN1, FGF) were down-regulated below 50% by Dycal.
CONCLUSIONS
Both wMTA and Dycal induced changes in gene expressions related with differentiation and proliferation of pulp cells. wMTA induced changes in gene expressions related with mineralization, and Dycal induced those related with angiogenesis. The genes related with inflammation were more expressed by Dycal than by wMTA. It was confirmed that both wMTA and Dycal were able to induce gene expression changes concerned with the pulp repair in different ways.

Keyword

Calcium hydroxide; Microarray; Mineral trioxide aggregate; Pulp capping; Pulp cell; Reverse transcriptase polymerase chain reaction

MeSH Terms

Aluminum Compounds
Calcium
Calcium Compounds
Calcium Hydroxide
Dental Pulp
Dental Pulp Capping
Drug Combinations
Gene Expression
Glutamates
Guanine
Humans
Hydroxides
Inflammation
Interleukin-11
Minerals
Oxides
Polytetrafluoroethylene
Reverse Transcriptase Polymerase Chain Reaction
RNA
RNA-Directed DNA Polymerase
Silicates
Transcriptome
Pemetrexed
Aluminum Compounds
Calcium
Calcium Compounds
Calcium Hydroxide
Drug Combinations
Glutamates
Guanine
Hydroxides
Interleukin-11
Minerals
Oxides
Polytetrafluoroethylene
RNA
RNA-Directed DNA Polymerase
Silicates

Figure

• 

  Figure 1. Classification of expressed genes by wMTA treatment. The genes which were up-or down-regulated by at least twofold following a cellular treatment with wMTA at any time point were classified using the panther classification system (http://www.pantherdb.org).

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  Figure 2. Classification of expressed genes by Dycal treatment. The genes which were up-or down-regulated by at least twofold following a cellular treatment with Dycal at any time point were classified using the panther classification system.

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