J Asthma Allergy Clin Immunol.
2001 Apr;21(2):223-230.
Role of IL-5-activated eosinophils on collagen gel contraction by lung fibroblasts
- Affiliations
-
- 1Department of Internal Medicine, Seoul National University College of Medicine. juinea@snu.ac.kr.
- 2Institute of Allergy and Clinical Immunology, Seoul National University Medical Research Center, Seoul, Korea.
Abstract
-
BACKGROUND: Subepithelial fibrosis plays a major role in the development of irreversible airway obstruction in asthma. Eosinophils are major effector cells in allergic inflammation, and it has been suggested that eosinophil-derived mediators such as transforming growth factor-beta (TGF-beta) play a role in the pathogenesis of airway remodeling.
OBJECTIVE
The aim of this study was to evaluate whether eosinophils activated by IL-5 plays a major role in the collagen gel contraction by lung fibroblasts. METHOD: Various cell numbers of lung fibroblasts were cultured in collagen gels to determine the appropriate numbers of fibroblasts. Purified human peripheral blood eosinophils were activated by IL-5 for 3 days, and TGF-beta mRNA expression was evaluated using semiquantitative RT- PCR. The cultured supernatants with or without TGF-beta were added to the collagen gel media with lung fibroblasts, and collagen gel diameter was serially measured to evaluate collagen gel contraction.
RESULTS
The amount of collagen gel contraction was significantly associated with the number of fibroblasts (p< 0.05), and TGF-beta significantly contracted the collagen gel to contract in a dose-dependent manner (p< 0.05). However, supernatants derived from IL-5-activated eosinophils did not contract the collagen gel compared to controls (p> 0.05). Moreover, expression of TGF-beta mRNA in eosinophils was the same before and after stimulus of IL-5.
CONCLUSION
Activated eosinophils by IL-5 may play a minor role in the collagen gel contraction by lung fibroblasts.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Collagen Gel Contraction by Cultured Fibroblasts Derived from Normal Skin, Oral Mucosa, and Hypertrophic Scar
- Simvastatin as a Modulator of Tissue Remodeling through Inhibition of Matrix Metalloproteinase (MMP) Release from Human Lung Fibroblasts
- Role of Hydrogen Sulfide in the Survival of Fibroblasts and Fibroblast-mediated Contraction of Collagen Gel
- Effect of Nitric Oxide on the Migration and Fibroblast-mediated Contraction of Collagen Gels
- The effect of the application of growth factors on wound contraction : An experimental study in a fibroblast-populated collagen lattice
