Korean J Otolaryngol-Head Neck Surg.  2006 Mar;49(3):297-302.

An Implant for Bone Formation using Autologous Mesenchymal Stem Cells

Affiliations
  • 1Department of Otorhinolaryngology & Head Neck Surgery, Yonsei University Wonju College of Medicine, Wonju, Korea. rhico@wonju.yonsei.ac.kr
  • 2FCB-Pharmicell, Seongnam, Korea.

Abstract

BACKGROUND AND OBJECTIVES: The aim of the current study was to examine ectopic bone formation with autologous mesenchymal stem cells (MSC) on a porous 3-D Poly (DL-lactic-co-glycolic acid, PLGA) in canine model. MATERIALS AND METHOD: Bone marrow derived autologous mesenchymal stem cells (MSCs) were isolated and purified from dogs. The porous PLGA scaffold loaded MSCs was treated with osteo-inductive medium containing dexamethasone, beta glycerophosphate and ascorbic acid. Osteogenic differentiation and mineralization were examined with RT-PCR for osteocalcin mRNA and Von Kossa staining of the cell-polymer complex. For in vivo bone formation, polymers containing osteogenic differentiated cells were implanted into the subperiosteal space of the canine maxillary sinus and evaluated at 1st and 2nd month
RESULTS
In vitro osteogenic differentiation was evident from 2 weeks after osteogenic induction. The mineralization was increased in a time dependent manner. Ectopic bone was generated in the subperiosteal space of canine maxillary sinus at 1 month and matured at 2 months.
CONCLUSION
From these results, we conclude that ectopic tissue engineered bone formation in the facial area is possible and that it may be useful for facial reconstruction or regeneration of bone defect.

Keyword

Tissue engineering; Mesenchymal Stem cells; Osteogenesis; Cell differentiation

MeSH Terms

Animals
Ascorbic Acid
Bone Marrow
Cell Differentiation
Choristoma
Dexamethasone
Dogs
Maxillary Sinus
Mesenchymal Stromal Cells*
Osteocalcin
Osteogenesis*
Polymers
Regeneration
RNA, Messenger
Tissue Engineering
Ascorbic Acid
Dexamethasone
Osteocalcin
Polymers
RNA, Messenger
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