J Korean Orthop Res Soc.  2008 Oct;11(2):72-83.

Comparison of Bone Formation between Allogenic Periosteum-Derived Mesenchymal Stem Cells and Cultured Osteoblasts Transplanted into Long-Bone Defects of Rabbits

• Kim HT
• Lee CB
• Youn MS
• Lee JS
• Jun ES
• Chang HK

Abstract

PURPOSE
To analyze the bone formation between allogenic periosteum-derived mesenchymal stem cells and cultured osteoblasts transplanted into rabbit long-bone defects.
MATERIALS AND METHODS
Forty white rabbits were grouped according to the material transplanted into their tibial bone defects: stem cells plus agar (group 1); osteoblasts plus agar (group 2); osteoblasts plus gelfoam (group 3). Bone formation was evaluated radiologically, histologically, and using serum osteoblastic markers during 8 weeks following transplantation.
RESULTS
Group 1 and 2 both showed satisfactory bone formation, with group 2 showing thicker and more abundant bone trabeculae than group 1. Rabbits in group 3 showed worse bone formation than group 1 and 2.
CONCLUSION
These results demonstrate that rabbits implanted with osteoblasts (with agar) showed more satisfactory bone formation than those inserted with stem cells; thus osteoblasts may be useful in the treatment of bone defects.

Keyword

Rabbit; Osteoblast; Bone formation

MeSH Terms

Agar
Gelatin Sponge, Absorbable
Humans
Mesenchymal Stromal Cells
Osteoblasts
Osteogenesis
Rabbits
Stem Cells
Transplants
Agar