Korean J Obstet Gynecol.  2004 Apr;47(4):618-626.

Comparison of Protein Expression in Normal Myometrium and Uterine Leiomyoma Using Two-Dimensional Gel Electrophoresis in Korean Women

• Lee SK
• Bae SM
• Kim KW
• Kim MS
• Park EK
• Kim YW
• Ro DY
• Lee JM
• Namkoong S
• Kim CK
• Ahn WS

Abstract

OBJECTIVE
Comparison of protein expression by two-dimensional gel electrophoresis (2-DE) in normal myometrium and uterine leiomyoma in Korean women.
METHODS
Normal myometrium and uterine leiomyoma tissues were solubilized with 2-DE buffer and the first dimension of PROTEAN IEF CELL, isoelectric focusing (IEF), was performed using pH4-8 linear IPG strips of 17 cm. And then running 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS- PAGE) and sliver stain. Scanned image analyzed using PDQuest 2-D softwareTM. Protein spot spectrum was identified by assisted laser desorption/ionization-time of fighting (MALDI-TOF) and the protein mass spectrums identification were performed by searching protein databases of Swiss-prot/TrEMBL, Mascot and MS-FIT.
RESULTS
In this study, we found 17 up-regulated proteins (phosphate carrier protein, 60 kDa heat shock protein, acidic calcium-independent, glutathione transferase omega, chloride intracellular channel 4, Ras-related protein Rab-11B, phosphatidylinositol transfer protein alpha isoform, type II keratin subunit protein, Cofilin 2 isoform 1, transgelin, ATP carrier protein, alpha-catenin homolog, parkinson disease 2, apo-cellular retinoic acid binding protein II, osteoglycin preproprotein, proteasome activator subunit 1 isoform, Unnamed protein) and 7 down-regulated proteins (Serum amyloid P component, annexin IV, alpha 1 actin precursor, hypoxanthine-guanine phosphoribosyltransferase, tumor necrosis factor receptor superfamily member EDAR precursor, peroxiredoxin 2, translation elongation factor EF-Tu precursor) between myometrium and leiomyoma.
CONCLUSION
2-DE offer total protein expression between normal myometrium and uterine leiomyoma, and searching of differently expressed protein for the diagnostic markers of leiomyoma.

Keyword

Cancer; Leiomyoma; Myometrium; Two-dimensional gel electrophoresis (2-DE); Matrix assisted laser desorption/ionization-time of flighting (MALDI-TOF)

MeSH Terms

Actins
Adenosine Triphosphate
alpha Catenin
Animals
Annexin A4
Carrier Proteins
Cofilin 2
Databases, Protein
Electrophoresis, Gel, Two-Dimensional*
Electrophoresis, Polyacrylamide Gel
Female
Glutathione Transferase
Heat-Shock Proteins
Humans
Hypoxanthine Phosphoribosyltransferase
Isoelectric Focusing
Keratins, Type II
Leiomyoma*
Mice
Myometrium*
Parkinsonian Disorders
Peptide Elongation Factor Tu
Peptide Elongation Factors
Peroxiredoxins
Phospholipid Transfer Proteins
Proteasome Endopeptidase Complex
Receptors, Tumor Necrosis Factor
Running
Serum Amyloid P-Component
Sodium Dodecyl Sulfate
Tretinoin
Actins
Adenosine Triphosphate
Annexin A4
Carrier Proteins
Cofilin 2
Glutathione Transferase
Heat-Shock Proteins
Hypoxanthine Phosphoribosyltransferase
Keratins, Type II
Peptide Elongation Factor Tu
Peptide Elongation Factors
Peroxiredoxins
Phospholipid Transfer Proteins
Proteasome Endopeptidase Complex
Receptors, Tumor Necrosis Factor
Serum Amyloid P-Component
Sodium Dodecyl Sulfate
Tretinoin
alpha Catenin