Korean J Obstet Gynecol.  2004 Jun;47(6):1086-1092.

Cloning and Expression Pattern of FSTL1 mRNA in Normal Myometrium and Uterine Leiomyoma

• Kim YS
• Kim JS
• Qian YR
• Li MH
• Kim JE
• Chun YY
• Kee KS
• Cheon HS
• Kim SJ

Abstract

OBJECTIVE
To study the influence and cloning of differentially expressed genes in human female normal myometrium and uterine leiomyoma tissue.
METHODS
In this experiment, human uterus tissues (n=25) were taken for total RNA isolation by using Trizol reagent. Differential display was performed by using GeneFishingTM DEG Kit and processed to cDNA sequencing and gene cloning for Follistatin-like 1 (FSTL1). Data were analyzed with the image Master VDS software and statistical significance was defined as p<0.05 by paired t test results.
RESULTS
FSTL1 mRNA expression level was significantly higher (p<0.05) in normal and adjacent normal myometrium tissues than uterine leiomyoma tissue of women in the reproductive age. Whereas in the menopausal age, FSTL1 mRNA expression level was significantly higher (p<0.05) in uterine leiomyoma than normal myometrium. There was no significant differences between uterine leiomyoma and adjacent normal myometrium.
CONCLUSION
Although the mechanisms of FSTL1 gene were uncertain, FSTL1 seemed to play an important role in the growth of uterine leiomyoma, it also might be related to the regulation of uterine leiomyoma growth inhibiting factors by modulating Follistatin related protein gene (FLRG) system.

Keyword

Follistatin-like 1 (FSTL1); Follistatin related protein gene (FLRG); Myometrium; Leiomyoma

MeSH Terms

Animals
Clone Cells*
Cloning, Organism*
DNA, Complementary
Female
Follistatin
Humans
Leiomyoma*
Mice
Myometrium*
RNA
RNA, Messenger*
Uterus
DNA, Complementary
Follistatin
RNA
RNA, Messenger