Korean J Nutr.  2004 Nov;37(9):763-770.

Effect of Dietary Conjugated Linoleic Acid (CLA) Isomers on Tumor Incidence and the Protein Expression of Cyclooxygenase-2 and Protein Kinase C in Colonic Mucosa of DMH-Treated Rats

• Park HS
• Chun CS
• Yoon JH

Abstract

This study was designed to compare the anti-carcinogenic effect of conjugated linoleic acid isomers on tumor incidence, cell proliferation and the levels of thromboxane (TX)B2, prostaglandin (PG)E2 and 1,2-diacylglycerol (DAG), and the related enzyme expression of cyclooxygenase (COX)-2 and protein kinase C (PKC) in colonic mucosa of 1,2-dimethylhydrazine (DMH)-treated rats. One hundred eight male Sprague Dawley rats were randomly divided into 3 groups depending on the types of CLA isomers, i.e. control group (no CLA contained), c9t11 group (cis-9, trans-11 CLA contained), and t10c12 group (trans-10, cis-12 CLA contained). The experimental diet was composed of protein at 20%, carbohydrate at 56.2%, and fat at 14.5% including 1.0% CLA isomers by weight. The experimental diet was fed for 30 weeks with the initiation of intramuscular injection of DMH, which was injected twice a week for 6 weeks to give total dose of 180 mg per kg body weight. Two CLA isomers (c9, t11; t10, c12) significantly reduced tumor incidence and cell proliferation by reducing the protein expression of COX-2 and PKC, and the level of TXB2, PGE2, and DAG in colonic mucosa. However, there was no significant difference in anti-carcinogenic effect between c9t11-CLA and t10c12-CLA.

Keyword

colon cancer; CLA isomers; cell proliferation; cyclooxygenase-2; protein kinase C

MeSH Terms

1,2-Dimethylhydrazine
Animals
Anticarcinogenic Agents
Body Weight
Cell Proliferation
Colon*
Colonic Neoplasms
Cyclooxygenase 2*
Diet
Dimenhydrinate
Dinoprostone
Humans
Incidence*
Injections, Intramuscular
Linoleic Acid*
Male
Mucous Membrane*
Prostaglandin-Endoperoxide Synthases
Protein Kinase C*
Protein Kinases*
Rats*
Rats, Sprague-Dawley
1,2-Dimethylhydrazine
Anticarcinogenic Agents
Cyclooxygenase 2
Dimenhydrinate
Dinoprostone
Linoleic Acid
Prostaglandin-Endoperoxide Synthases
Protein Kinase C
Protein Kinases