Ann Dermatol.  2015 Apr;27(2):121-127. 10.5021/ad.2015.27.2.121.

Retinoic Acid Promotes Interleukin-4 Plasmid-Dimethylsulfoxide Topical Transdermal Delivery for Treatment of Psoriasis

Affiliations
  • 1State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, China. lijionghh@sohu.com

Abstract

BACKGROUND
Psoriasis is an autoimmune disease that is caused by a shift in the Th1/Th2 balance toward Th1-dominant immunity. It has been established as an effective treatment to counteract psoriasis by subcutaneous injection of recombinant interleukin (IL)-4, and IL-4 gene therapy by topical transdermal penetration has shown its antipsoriatic effect in mice. Retinoic acid (RA) and dimethylsulfoxide can increase the efficiency of gene transfection in the topical transdermal delivery system.
OBJECTIVE
We investigated whether RA could improve anti-psoriasis efficiency using IL-4 expression plasmid pORF-mIL-4 (pIL-4) via transdermal delivery system in K14-vascular endothelial growth (K14-VEGF) factor transgenic mice.
METHODS
After pretreatment with RA, plasmid pIL-4 in 10% dimethylsulfoxide was applied to the ear skin by topical transdermal penetration. Hematoxylin- eosin staining and immunohistochemistry were performed with ear samples to evaluate anti-psoriasis efficiency in mice.
RESULTS
The psoriasis pathological features were relieved and psoriasis-associated factors were significantly reduced.
CONCLUSION
Our results reveal that topical application of pIL-4 in dimethylsulfoxide by transdermal delivery with RA pretreatment can improve psoriasis significantly.

Keyword

Dimethyl sulfoxide; Gene therapy; Interleukin-4; Psoriasis; Retinoic acid; Transdermal

MeSH Terms

Animals
Autoimmune Diseases
Dimethyl Sulfoxide
Ear
Eosine Yellowish-(YS)
Genetic Therapy
Immunohistochemistry
Injections, Subcutaneous
Interleukin-4*
Interleukins
Mice
Mice, Transgenic
Plasmids
Psoriasis*
Skin
Transfection
Tretinoin*
Dimethyl Sulfoxide
Eosine Yellowish-(YS)
Interleukin-4
Interleukins
Tretinoin
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