Korean J Nephrol.
2008 Jan;27(1):4-12.
The Anti-apoptotic Effect of Adrenomedullin on Vascular Endothelial Cells in Radiocontrast Media-induced Apoptosis
- Affiliations
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- 1Department of Internal Medicine, Chonbuk National University Medical School, Jeonju, Korea. kwon@chonbuk.ac.kr
- 2Research Institute of Clinical Medicine, Chonbuk National University Medical School, Jeonju, Korea.
- 3Food function Research Division, Korea Food Research Institute, Sungnam, Republic of Korea.
Abstract
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PURPOSE: Because vascular endothelial cells play a pivotal role in the vascular diseases, damage of vascular endothelial cells lead to progression of vascular disease. Apoptotic damage of cells is an important mechanism in vascular disease. Therefore, several growth factors that have antiapoptotic effect may have a protective role in maintaining a cell function in apoptotic cell injury. In this study, we examined the effects of adrenomedullin on apoptosis in iopromide-induced endothelial cell injury.
METHODS
Human umbilical vein endothelial cells were incubated with nonionic radiocontrast agent, iopromide and/or adrenomedullin. Apoptosis was assessed quantitatively using FACScan after annexin V-FITC and propidium iodide staining, and by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) stain. Signaling pathway was evaluated by Western blot analysis of phospho-Akt and Akt.
RESULTS
Iopromide-induced apoptosis in human umbilical vein endothelial cells was increased in a dose-dependent manner. Adrenomedullin prevented iopromide-induced apoptosis in human umbilical vein endothelial cells in a dose dependent manner. Wortmannin, phosphatidylinositol 3-kinase inhibitor, decrease the adrenomedullin-induced antiapoptotic effect.
CONCLUSION
These results suggest that adrenomedullin protects vascular endothelial cells from iopromide-induced apoptosis by regulating the activity of Akt.
Keyword
MeSH Terms
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Adrenomedullin
Androstadienes
Apoptosis
Blotting, Western
Contrast Media
Endothelial Cells
Human Umbilical Vein Endothelial Cells
Intercellular Signaling Peptides and Proteins
Iohexol
Phosphatidylinositol 3-Kinase
Propidium
Vascular Diseases
Adrenomedullin
Androstadienes
Contrast Media
Intercellular Signaling Peptides and Proteins
Iohexol
Phosphatidylinositol 3-Kinase
Propidium
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