Cancer Res Treat.  2006 Feb;38(1):48-53.

Resveratrol at High Doses Acts as an Apoptotic Inducer in Endothelial Cells

Affiliations
  • 1Department of Molecular Biology & the Institute of Nanosensor and Biotechnology, Dankook Univiersity, Seoul, Korea. heonyong@dankook.ac.kr

Abstract

PURPOSE
S: Resveratrol is a phenolic compound found in grapes and other food products. In order to assess the availability of resveratrol as an angio-inhibiting drug, we examined whether resveratrol plays an important role in bovine aortic endothelial cells (BAECs) for cell apoptosis and cell migration.
METHODS
AND MATERIALS: Endothelial cell apoptosis was observed as detected by the Hoechst staining and the caspase-3 activity. Additionally, Western blotting was performed for monitoring the activities of various cell signaling molecules.
RESULTS
Resveratrol was shown to act as a pro-apoptotic agent. The pro-apoptotic effect of resveratrol was as great as that of etoposide, a well-known anti-cancer drug. In addition, resveratrol had an inhibitory effect on endothelial cell migration. The demonstrated efficacy of resveratrol suggests that resveratrol may be utilized as an anti-angiogenic drug. To determine the underlying mechanisms, we further investigated which signaling molecules are activated by resveratrol. Extracellular signal-regulated kinase (ERK) was activated by the treatment with resveratrol in BAECs, whereas endothelial nitric oxide synthetase (eNOS), Akt, and Jun N-terminal kinase (JNK) were inhibited. The pretreatment with PD compound, an ERK inhibitor, had no effect on the pro-apoptosis induced by resveratrol.
CONCLUSION
Resveratrol plays an important role in endothelial cell apoptosis, indicating that resveratrol can be utilized as a potent anti-angiogenic drug.

Keyword

Resveratrol; Endothelial cells; Apoptosis; Caspase-3; Cell signaling

MeSH Terms

Apoptosis
Blotting, Western
Caspase 3
Cell Movement
Endothelial Cells*
Etoposide
Nitric Oxide Synthase
Phenol
Phosphotransferases
Vitis
Caspase 3
Etoposide
Nitric Oxide Synthase
Phenol
Phosphotransferases
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