- A Rapid and Simple flow Cytometric Method for Measuring Cell Viability Using Propidium Iodide Staining and Forward Scatter Measurement
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Lee YS, Youn SW, Kim KH, Park KC
- KMID: 2360489
- Ann Dermatol.
- 1996 Jul;8(3):195-200.
- doi: 10.5021/ad.1996.8.3.195
BACKGROUND: The importance of the determination of cell viability has prompted the development of several assays of viability that utilize the exclusion of certain dyes by viable cell membranes. Recently,... -
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- Effects of Exposure of Propidium Iodide and Bisbenzimide on Differential Staining of Mouse Blastocysts
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Park KS, Park SB, Lee TH, Chun SS, Song HB
- KMID: 1566329
- Korean J Fertil Steril.
- 2002 Dec;29(4):317-322.
OBJECTIVE: These experiments were conducted to investigate the optimal expose length of propidium iodide (PI) and bisbenzimide on differential staining of mouse blastocysts. MATERIALS AND METHODS: A total 964 blastocysts (early~hatched)... -
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- Evaluation of Propidium Monoazide Real-Time PCR for Viablity of Mycobacterium leprae
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Kim JP, Kim YS, Kim CW
- KMID: 2369142
- Korean Lepr Bull.
- 2016 Dec;49(1):13-22.
- doi: 10.0000/klb.2016.49.1.13
BACKGROUND: Conventional acid-fast bacilli (AFB) staining cannot differentiate viable from dead cells. Propidium monoazide (PMA) is a photoreactive DNA-binding dye that inhibits PCR amplification by DNA modification. OBJECT: The author evaluated... -
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- Selective detection of viable Enterococcus faecalis using propidium monoazide in combination with real-time PCR
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Kim S, Lee S, Kim E, Seo D, Song Y, Jung I
- KMID: 1428464
- J Korean Acad Conserv Dent.
- 2008 Nov;33(6):537-544.
- doi: 10.5395/JKACD.2008.33.6.537
Polymerase chain reaction (PCR) can detect bacteria more rapidly than conventional plate counting. However DNA-based assays cannot distinguish between viable and dead cells due to persistence of DNA after cells... -
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- Relationship between Chromatin Pattern of Tumor Cell Nuclei and Transcriptional Sites
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Juhng SW
- KMID: 2172304
- Chonnam Med J.
- 2005 Dec;41(3):253-258.
Malignant cell nuclei, in general, have increased amount of heterochromatin and decreased electron density of euchromatin, making the chromatin pattern coarser than that of benign cell nuclei. Chromatin pattern in... -
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- An analysis of the flow cytometry in transitional cell carcinoma of bladder
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Kwon SD, Cho JH
- KMID: 2060295
- Korean J Urol.
- 1991 Oct;32(5):704-710.
The 32 patients with transitional cell carcinoma of the bladder underwent flow cytometric analysis or nuclei extracted from paraffin-embedded archival material and isolated nuclei were stained with propidium iodide. The... -
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- Effect of temperature and medium on the viability of Mycobacterium leprae during long term-storage
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Park JH, Kim YJ, Kim JP
- KMID: 2466491
- Korean Lepr Bull.
- 2019 Dec;52(1):41-50.
- doi: 10.33161/klb.2019.52.1.41
BACKGROUND: Mycobacterium leprae (M. leprae) is pathogenic bacterium with polymorphic, acid-fast properties and causes leprosy that it is called Hansen's disease. Leprosy can be completely cured using multidrug therapy (MDT),... -
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- Ultraviolet B-Induced Apoptosis of Normal Human Melanocytes and G361 Cells
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Kim YG, Hwang JH, Ahn JS, Kim KH, Youn JI, Kim JA, Chun DK
- KMID: 2359780
- Ann Dermatol.
- 1998 Jul;10(3):147-152.
- doi: 10.5021/ad.1998.10.3.147
BACKGROUND: Ultraviolet(UV) light is one of the injurious environmental agents which is known to lead to apoptosis of cells. However, studies on UVB-induced apoptosis of melanocytes are still lacking and... -
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- Infected with Enterovirus
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Kim DS
- KMID: 2335475
- J Korean Pediatr Soc.
- 2000 Mar;43(3):372-379.
PURPOSE: To evaluate apoptosis of cord blood mononuclear cells (CBMC) infected with enterovirus. MEHTODS: Cord blood was obtained from 20 neonates which were delivered vaginally without any complications. Cord blood mononuclear... -
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- Flow Cytometric Method for Counting Residual Leukocytes
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Choi KL, Cho D, Shin JH, Suh SP, Ryang DW
- KMID: 2089989
- Korean J Blood Transfus.
- 2004 Jun;15(1):1-7.
BACKGROUND: Simple manual method using a Nageotte hemocytometer counting residual leukocytes [White blood cells(WBC)] in blood components is subjective, labor-intensive, time consuming and variable within and between laboratories. The aim... -
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- Neurotoxic Effect of beta-Amyloid Peptide in Hippocampal Slice Culture
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Park JS, Jo MS, Jung YJ, Lee KE
- KMID: 2352867
- Ewha Med J.
- 2003 Jun;26(2):143-148.
- doi: 10.12771/emj.2003.26.2.143
Alzheimer's disease(AD) is primarily characterized by neurofibrillary tangles, senile plaques, and neurodegeneration. The major component of senile plaques is the beta-amyloid peptide(A beta), Which is considered to have a causal... -
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- Non-homologous End Joining Inhibitor SCR-7 to Exacerbate Low-dose Doxorubicin Cytotoxicity in HeLa Cells
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Kumar A, Bhatkar D, Jahagirdar D, Sharma NK
- KMID: 2375548
- J Cancer Prev.
- 2017 Mar;22(1):47-54.
- doi: 10.15430/JCP.2017.22.1.47
Among the genotoxic drug regimens, doxorubicin (DOX) is known for its high-dose side effects in several carcinomas, including cervical cancer. This study reports on testing the combined use of a... -
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- Differences in Their Proliferation and Differentiation between B-1 and B-2 Cell
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Yeo SG, Cha CI, Park DC
- KMID: 2167982
- Immune Netw.
- 2006 Mar;6(1):1-5.
- doi: 10.4110/in.2006.6.1.1
BACKGROUND: B cell subset has been divided into B-1 cells and B-2 cells. B-1 cells are found most prominently in the peritoneal cavity, as well as constituting a small proportion... -
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- The Role of Nitric Oxide (NO) in UVB-induced Apoptosis in HaCaT Keratinocytes
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Sung KJ, Paik EM, Kim MJ, Suh CW, Suh HS, Choi JH
- KMID: 2116065
- Korean J Dermatol.
- 1999 Nov;37(11):1596-1602.
BACKGROUND: According to target cells, apoptosis-inducing agents, and NO concentration, NO concentration, NO shows both pro- and antiapoptotic effects. OBJECTS: Our study was perfermed to verify the role of NO... -
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- Apoptosis of Cultured Rabbit Keratocyte Induced by Mitomycin-C
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Kim MK, Wee WR, Kim MS, Park IS, Lee JH
- KMID: 2205750
- J Korean Ophthalmol Soc.
- 2000 Feb;41(2):332-338.
To determine whether mitomycin-C[MMC]treatment induces apoptosis in cultured keratocytes. Cultured rabbit keratocytes were exposed to MMC[up to 0.4 milligram/milliliter]or phosphate-buffered saline[PBS]for 5 minutes. Light and transmission electron microscopic examination, DNA... -
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- Relationship between Radiation Induced Activation of DNA Repair Genes and Radiation Induced Apoptosis in Human Cell Line A431
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Bom HS, Min JJ, Choi KH, Kim KK
- KMID: 2078705
- Korean J Nucl Med.
- 2000 Apr;34(2):144-153.
PURPOSE: The purpose of this study was to evaluate the relationship between radiation-induced activation of DNA repair genes and radiation induced apoptosis in A431 cell line. MATERALS AND METHODS: Five and 25... -
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- Regulation of Astroglial Volume by Ketamine in Glutamate Induced Cellular Volume Changes
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Kim MH, Hahm TS, Ahn HJ
- KMID: 2299243
- Korean J Anesthesiol.
- 1997 Dec;33(6):1005-1011.
- doi: 10.4097/kjae.1997.33.6.1005
BACKGROUND: Relative changes of astroglial volume constitute the major part of brain edema, which is related to delayed neuronal damage. Several factors including glutamate may contribute to astroglial swelling. Intravenous... -
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- In Vitro Antifungal Activity of (1)-N-2-Methoxybenzyl-1,10-phenanthrolinium Bromide against Candida albicans and Its Effects on Membrane Integrity
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Setiawati , Nuryastuti T, Ngatidjan , Mustofa M, Jumina J, Fitriastuti D
- KMID: 2376161
- Mycobiology.
- 2017 Mar;45(1):25-30.
- doi: 10.5941/MYCO.2017.45.1.25
Metal-based drugs, such as 1,10-phenanthroline, have demonstrated anticancer, antifungal and antiplasmodium activities. One of the 1,10-phenanthroline derivatives compounds (1)-N-2-methoxybenzyl-1,10-phenanthrolinium bromide (FEN), which has been demonstrated an inhibitory effect on the... -
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- Susceptibility of rat hippocampal neurons to hypothermia during development
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Seo KA, Kim S, Lee NM, Chae SA
- KMID: 1712452
- Korean J Pediatr.
- 2013 Oct;56(10):446-450.
- doi: 10.3345/kjp.2013.56.10.446
PURPOSE: This study evaluated the extent of damage due to hypothermia in the mature and immature brain. METHODS: Hippocampal tissue cultures at 7 and 14 days in vitro (DIV) were used... -
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- Viability of Cells in Aspirated Fat Tissue after 1 Year Cryopreservation
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Son D, Oh J, Choi T, Kim J, Han K
- KMID: 2120359
- J Korean Soc Plast Reconstr Surg.
- 2009 Mar;36(2):135-139.
PURPOSE: The use of an autogenous fat graft has become a common procedure in plastic surgery. However, questions remain concerning on the viability of fat cells and preservation method of... -
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