Ewha Med J.  2003 Jun;26(2):143-148. 10.12771/emj.2003.26.2.143.

Neurotoxic Effect of beta-Amyloid Peptide in Hippocampal Slice Culture

• Park JS
• Jo MS
• Jung YJ
• Lee KE

• KMID: 2352867
• DOI: http://doi.org/10.12771/emj.2003.26.2.143

Abstract

Alzheimer's disease(AD) is primarily characterized by neurofibrillary tangles, senile plaques, and neurodegeneration. The major component of senile plaques is the beta-amyloid peptide(A beta), Which is considered to have a causal role in AD. However, the biological activities of Abeta in AD has not been clearly defined. In this study we have investigated the effects of Abeta 25-35 fragment to neurons using organotypic hippocampal slice culture system which maintained intact hippocampal synaptic circuit and anatomy. Hippocampal slice culture is prepared from rat postnatal 10-old days and after 14 days culture, slices were treated with 10uM Abeta 25-35 fragment. Neuronal death was measured with propidium iodide(PI) uptake and NeuN, neuronal marker, staining. After treatment of Abeta 25-35 fragment for 3days or 7days on hippocampal slice culture, we observed the increased PI uptake and the decreased number of NeuN-stained neuron in CA1 region of hippocampal pyramidal layer or dentate gyrus. These results suggested that Abeta 25-35 fragment exerts the neurotoxicity in hippocampal slice culture.

Keyword

Beta-Amyloid peptide; Neurotoxicity; Hippocampal slice culture

MeSH Terms

Animals
Dentate Gyrus
Neurofibrillary Tangles
Neurons
Plaque, Amyloid
Propidium
Rats
Propidium