Immune Netw.  2006 Mar;6(1):1-5. 10.4110/in.2006.6.1.1.

Differences in Their Proliferation and Differentiation between B-1 and B-2 Cell

• Yeo SG
• Cha CI
• Park DC

• KMID: 2167982
• DOI: http://doi.org/10.4110/in.2006.6.1.1

Abstract

BACKGROUND: B cell subset has been divided into B-1 cells and B-2 cells. B-1 cells are found most prominently in the peritoneal cavity, as well as constituting a small proportion of splenic B cells and they are larger and less dense than B-2 cells in morphology. This study was designed to compare the differences in their proliferation and differentiation between B-1 and B-2 cell.
METHODS
We obtained sorted B-1 cells from peritoneal fluid and B-2 cells from spleens of mice. Secreted IgM was measured by enzyme-linked immunosorbent assay. Entering of S phase in response to LPS-stimuli was measured by proliferative assay. Cell cycle analysis by propidium iodide was performed. p21 expression was assessed by real time PCR.
RESULTS
Cell proliferation and cell cycle progression in B-1 and B-2 cells, which did not occur in the absence of LPS, required LPS stimulation. After LPS stimulation, B-1 and B-2 cells were shifted to S and G2/M phases. p21 expression by resting B-1 cells was higher than that of resting B-2 cells.
CONCLUSION
B-1 cells differ from conventional B-2 cells in proliferation, differentiation and cell cycle.

Keyword

B-1 cell; B-2 cell; immunology; differentiation; cell cycle; p21

MeSH Terms

Allergy and Immunology
Animals
Ascitic Fluid
B-Lymphocytes
Cell Cycle
Cell Proliferation
Enzyme-Linked Immunosorbent Assay
Immunoglobulin M
Mice
Peritoneal Cavity
Propidium
Real-Time Polymerase Chain Reaction
S Phase
Spleen
Immunoglobulin M
Propidium