Soonchunhyang Med Sci.  2013 Dec;19(2):93-98.

Effects of Ascorbic Acid on Osteoblast Differentiation in MC3T3-E1 Cells

• Yang HM
• Seo HS

Abstract

OBJECTIVE
Vitamin C is an essential agent for cell differentiation for cell. However, for osteogenic differentiation a combined medium of vitamin C, phosphoglyceride and dexamethasone is used. Individual effect of vitamin C can be observed in terms of cell growth and proliferation and eventual differentiation to assess the effect of this chemical agent for using as bone growth. This work investigates the dose dependent effect of vitamin C on MC3T3-E1 type pre-osteoblast cell on cell proliferation and differentiation.
METHODS
To investigate the dose dependent effect of vitamin C on MC3T3-E1 type pre-osteoblast cell predetermined amount of vitamin C was added to the medium and the medium was used for cell culture and cell differentiation. Relative cell viability and cell proliferation were checked to see the effect of vitamin C. Effect of dose dependent gene expression was carried out by reverse transcription-polymerase chain reaction with osteogenic differentiation specific genes.
RESULTS
Cell viability and cell proliferation was increased in all culture time. The pre-osteoblast cells show significantly higher expression of osteoblast formation specific gene alkaline phosphatase, osteocalcin, and osteopontin at a higher dose of vitamin C.
CONCLUSION
Higher doses of vitamin C with 50 microg/uL could significantly enhance the cell proliferation.

Keyword

MC3T3-E1; Ascorbic acid; Osteogenic

MeSH Terms

Alkaline Phosphatase
Ascorbic Acid*
Bone Development
Cell Culture Techniques
Cell Differentiation
Cell Proliferation
Cell Survival
Dexamethasone
Gene Expression
Osteoblasts*
Osteocalcin
Osteopontin
Alkaline Phosphatase
Ascorbic Acid
Dexamethasone
Osteocalcin
Osteopontin