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Aqueous extract of Petasites japonicus leaves promotes osteoblast differentiation via up-regulation of Runx2 and Osterix in MC3T3-E1 cells

Kim EJ, Jung JI, Jeon YE, Lee HS

BACKGROUND/OBJECTIVES: Petasites japonicus Maxim (P. japonicus) has been used as an edible and medicinal plant and contains many bioactive compounds. The purpose of this study is to investigate the effect...
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Zinc modulation of osterix in MC3T3-E1 cells

Seo HJ, Jeong JB, Cho YE, Kwun IS

Purpose: Zinc is known to be associated with osteoblast proliferation and differentiation. Osterix as zinc-finger transcription factor is also related to osteoblast differentiation and bone formation. In the present study, we...
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Anti-Oral Microbial Activity and Anti-Inflammatory Effects of Rosmarinic Acid in Lipopolysaccharide-Stimulated MC3T3-E1 Osteoblastic Cells on a Titanium Surface

Jeong MJ, Lim DS, Heo K, Jeong SJ

Background: The purpose of this study was to investigate the anti-oral microbial activity and anti-inflammatory effects of rosmarinic acid (RA) in lipopolysaccharide (LPS)-stimulated MC3T3-E1 osteoblastic cells on a titanium (Ti)...
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Glycyrrhiza uralensis (licorice) extracts increase cell proliferation and bone marker enzyme alkaline phosphatase activity in osteoblastic MC3T3-E1 cells

Cho YE, Kwun IS

PURPOSE: The Glycyrrhiza uralensis species (Leguminosae) as a medicinal biocompound, and one of its root components, isoliquritigenin (ISL), which is a flavonoid, has been reported to have anti-tumor activity in...
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Zinc upregulates bone-specific transcription factor Runx2 expression via BMP-2 signaling and Smad-1 phosphorylation in osteoblasts

Cho YE, Kwun IS

PURPOSE: Runx2 (runt-related transcription factor 2), a bone-specific transcription factor, is a key regulator of osteoblast differentiation and its expression is induced by the activation of BMP-2 signaling. This study...
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Hydroxyapatite Nanorod-Modified Sand Blasted Titanium Disk for Endosseous Dental Implant Applications

Park SJ, Kim BS, Gupta KC, Lee DY, Kang IK

BACKGROUND: Sand blasted titanium (Ti) is commonly used in designing endosseous dental implants due to its biocompatibility and ability to form bonds with bone tissues. However, titanium implants do not...
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Icaritin, a Flavonoid Derived from the Herb Epimedium, Promotes Osteogenic Differentiation of MC3T3-E1 Cells

Park DB, Lee HS, Ko SH

Osteoporosis is a metabolic bone disease that is characterized by low bone mass resulting from an increase in bone resorption relative to bone formation. The most current therapies for osteoporosis...
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Comparison of alkaline phosphatase activity of MC3T3-E1 cells cultured on different Ti surfaces: modified sandblasted with large grit and acid-etched (MSLA), laser-treated, and laser and acid-treated Ti surfaces

Li LJ, Kim SN, Cho SA

PURPOSE: In this study, the aim of this study was to evaluate the effect of implant surface treatment on cell differentiation of osteoblast cells. For this purpose, three surfaces were...
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Synergistic Effects of Chios Gum Mastic Extract and Low Level Laser Therapy on Osteoblast Differentiation

Lee KH, Kim YS, Yu SB, Kang HM, Kwak HH, Kim IR, Park BS

In the present study, we evaluated the effect of CGM on osteogenic differentiation of cultured osteoblasts, and determined whether combination treatment with LLLT had synergistic effects on osteogenic differentiation. The results...
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Effects of Scytosiphon lomentaria on osteoblastic proliferation and differentiation of MC3T3-E1 cells

Park MH, Kim S, Cheon J, Lee J, Kim BK, Lee SH, Kong C, Kim YY, Kim M

BACKGROUND/OBJECTIVES: Bone formation and bone resorption continuously occur in bone tissue to prevent the accumulation of old bone, this being called bone remodeling. Osteoblasts especially play a crucial role in...
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Anthraquinone Glycoside Aloin Induces Osteogenic Initiation of MC3T3-E1 Cells: Involvement of MAPK Mediated Wnt and Bmp Signaling

Pengjam Y, Madhyastha H, Madhyastha R, Yamaguchi , Nakajima Y, Maruyama M

Osteoporosis is a bone pathology leading to increased fracture risk and challenging the quality of life. The aim of this study was to evaluate the effect of an anthraquinone glycoside,...
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Nitric Oxide-Induced Autophagy in MC3T3-E1 Cells is Associated with Cytoprotection via AMPK Activation

Yang JY, Park MY, Park SY, Yoo HI, Kim MS, Kim JH, Kim WJ, Jung JY

Nitric oxide (NO) is important in the regulation of bone remodeling, whereas high concentration of NO promotes cell death of osteoblast. However, it is not clear yet whether NO-induced autophagy...
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The effects of bone morphogenetic protein-2 and enamel matrix derivative on the bioactivity of mineral trioxide aggregate in MC3T3-E1cells

Jeong Y, Yang W, Ko H, Kim M

  • KMID: 2316893
  • Restor Dent Endod.
  • 2014 Aug;39(3):187-194.
OBJECTIVES: The effects of bone morphogenetic protein-2 (BMP-2) and enamel matrix derivative (EMD) respectively with mineral trioxide aggregate (MTA) on hard tissue regeneration have been investigated in previous studies. This...
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Magnesium vs. machined surfaced titanium - osteoblast and osteoclast differentiation

Kwon YD, Lee DW, Hong SO

PURPOSE: This study focused on in vitro cell differentiation and surface characteristics in a magnesium coated titanium surface implanted on using a plasma ion source. MATERIALS AND METHODS: 40 commercially made...
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Effects of Ascorbic Acid on Osteoblast Differentiation in MC3T3-E1 Cells

Yang HM, Seo HS

  • KMID: 2317709
  • Soonchunhyang Med Sci.
  • 2013 Dec;19(2):93-98.
OBJECTIVE: Vitamin C is an essential agent for cell differentiation for cell. However, for osteogenic differentiation a combined medium of vitamin C, phosphoglyceride and dexamethasone is used. Individual effect of...
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Zinc may increase bone formation through stimulating cell proliferation, alkaline phosphatase activity and collagen synthesis in osteoblastic MC3T3-E1 cells

Seo HJ, Cho YE, Kim T, Shin HI, Kwun IS

  • KMID: 2313466
  • Nutr Res Pract.
  • 2010 Oct;4(5):356-361.
Zinc is an essential trace element required for bone formation, however not much has been clarified yet for its role in osteoblast. We hypothesized that zinc would increase osteogenetic function...
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Effects of 2-deoxy-D-glucose and quercetin on the gene expression of bone sialoprotein and osteocalcin during the differentiation in irradiated MC3T3-E1 osteoblastic cells

Lee JU, Kim KA, Koh KJ

  • KMID: 2034179
  • Korean J Oral Maxillofac Radiol.
  • 2009 Sep;39(3):121-132.
PURPOSE: To investigate the effects of 2-deoxy-D-glucose (2-DG) and quercetin (QCT) on gene expression of bone sialoprotein (BSP) and osteocalcin (OC) during the differentiation in irradiated MC3T3-E1 osteoblastic cells. MATERIALS AND...
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Effects of irradiation on TGF-beta1 mRNA expression and calcific nodule formation in MC3T3-E1 osteoblastic cell line

Song JS, Kim KA, Koh KJ

  • KMID: 1483931
  • Korean J Oral Maxillofac Radiol.
  • 2008 Sep;38(3):125-132.
PURPOSE: To investigate the effects of irradiation on transforming growth factor beta1 (TGF-beta1) mRNA expression and calcific nodule formation in MC3T3-E1 osteoblastic cell line. MATERIALS AND METHODS: Cells were cultured in...
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Cellular attachment and gene expression of osteoblast-like cells on zirconia ceramic surfaces

Pae A, Lee H, Kim HS, Baik J, Woo YH

  • KMID: 2118753
  • J Korean Acad Prosthodont.
  • 2008 Jun;46(3):227-237.
STATEMENT OF PROBLEM: Zirconium oxide can be a substitute to titanium as implant materials to solve the esthetic problems of dark color in the gingival portion of implant restorations....
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Cellular Zn depletion by metal ion chelators (TPEN, DTPA and chelex resin) and its application to osteoblastic MC3T3-E1 cells

Cho YE, Lomeda RA, Ryu SH, Lee JH, Beattie JH, Kwun IS

  • KMID: 2313289
  • Nutr Res Pract.
  • 2007 Mar;1(1):29-35.
Trace mineral studies involving metal ion chelators have been conducted in investigating the response of gene and protein expressions of certain cell lines but a few had really focused on...
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