Korean J Fertil Steril.  2002 Dec;29(4):317-322.

Effects of Exposure of Propidium Iodide and Bisbenzimide on Differential Staining of Mouse Blastocysts

• Park KS
• Park SB
• Lee TH
• Chun SS
• Song HB

Abstract

OBJECTIVE
These experiments were conducted to investigate the optimal expose length of propidium iodide (PI) and bisbenzimide on differential staining of mouse blastocysts. MATERIALS AND
METHODS
A total 964 blastocysts (early~hatched) was exposed to PI (n=831) (group I: RESULTS
In case of PI exposure, differential staining rates were significantly higher (p<0.05) in group I (89.8%) than in any others (group II: 77.6%; III: 29.6%; IV: 22.2%) and higher (p<0.05) in group II than in group III and IV. In case of bisbenzimide exposure, differential staining rates were not statistically differences in three groups (group A: 97.4%; B: 87.8%; C: 93.3%).
CONCLUSION
The differential staining rates of mouse blastocysts are not affected by the exposure length of bisbenzimide. However, blastocysts were exposed to PI with period of shorter than 15 sec show best outcomes of differential staining rates.

Keyword

Mouse; Blastocyst; Propidium iodide; Bisbenzimide; Differential staining

MeSH Terms

Animals
Bisbenzimidazole*
Blastocyst*
Mice*
Propidium*
Bisbenzimidazole
Propidium