Korean J Obstet Gynecol.  2009 Mar;52(3):336-345.

Effect of the decidualization of human endometrial cells on the hatching rates of the vitrified and the fresh mouse blastocysts

Affiliations
  • 1Department of Obstetrics and Gynecology, School of Medicine, Pusan National University, Busan, Korea. kuslee@pusan.ac.kr
  • 2Clinic of Infertility, Pusan National University Hospital, Busan, Korea.

Abstract


OBJECTIVE
To compare the hatching rates of the vitrified and the fresh mouse blastocysts co-cultured with decidualized or non decdualized human endometrial cells and to confirm the necessity of assisted hatching in the vitrified mouse blastocyst.
METHODS
Stromal and epitherial cells isolated from human endometrial tissue were co-cultured and decidualized with TGF-beta 1 and progesterone. The vitrified and the fresh mouse blastocysts were co-cultured with human decidualized or non-decidualized endometrial cells, respectively and the hatching rates were investigated.
RESULTS
Epithelial and stromal cells isolated from endometrial tissue were cultured seperately for 24 hours and stained by immunohistochemical staining for cytokeratin (epithelial cells) or vimentin (stromal cells). The immunohistochemical study was positive for cytokeratin or vimentin and confirmed that epithelial and stromal cells were isolated from endometrial tissue successfully. The co-cultured human stromal and epitherial cells were decidualized by administration of TGF-beta 1 and progesterone. The hatching rates of the fresh and the vitrified mouse blastocysts co-cultured with decidualized human endometrial cells were 89% and 31%, respectively. The hatching rates of the fresh and the vitrified mouse blastocysts co-cultured with non-decidualized human endometrial cells were 82% and 27%, respectively. The hatching rates were significantly higher in fresh mouse blastocysts than in vitrified mouse blastocysts regardless of decidualization of human endometrial cells (P<0.05).
CONCLUSION
The hatching rate was significantly higher in fresh mouse blastocysts than in vitrified mouse blastocysts. This results showed that the cryopreservation procedure caused the zona hardening of mouse blastocyst and the decidualization of endometrial cells did not affect to hatching rate of the vitrified mouse blastocysts. We confirmed that assisted hatching was necessary for improving the hatching rate of cryopreserved mouse blastocysts.

Keyword

Decidualization; Blastocyst; Co-culture; Hatching

MeSH Terms

Animals
Blastocyst
Coculture Techniques
Cryopreservation
Herpes Zoster
Humans
Keratins
Mice
Progesterone
Stromal Cells
Transforming Growth Factor beta
Vimentin
Keratins
Progesterone
Transforming Growth Factor beta
Vimentin
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