Korean J Gynecol Oncol.  2007 Sep;18(3):219-226.

A differential gene expression profiles by cDNA microarrays in endometrioid endometrial carcinoma: a preliminary study

• Chung MJ
• Chung EJ
• Lee TH
• Cho YL
• Park IS
• Lee YS

Abstract

OBJECTIVE
Endometrial carcinoma is the most common gynecological malignant disease in industrialized countries. However, the molecular bases for endometrial tumoriogenesis are not clearly elucidated. Our hypothesis is that there may be some difference in gene expression patterns between normal endometrium and endometrial cancer lesion. In this study, we analyzed the difference of gene expression profile with cDNA microarray.
METHODS
Normal endometrial tissues and cancer lesions were gathered from three patient with endometrioid endometrial cancer. cDNA microarray technique (KNU 4.8K chip) was applied to screen the different gene expression profiles.
RESULTS
Many genes such as interleukin-1 receptor-associated kinase 1 (IRAK1), bifunctional apoptosis regulator (BFAR), paraneoplastic antigen MA2 (PNMA2), zinc finger protein 257 (ZNF257), ras homolog gene family, member F (in filopodia) (ARHF), cell division cycle 27 (CDC27) were over-expressed in the endometrial cancer tissue. The genes were down-regulated in the endometrial cancer samples included fibronectin 1 (FN1), meiotic checkpoint regulator (MCPR), transforming growth factor beta-stimulated protein TSC-22 (TSC22), programmed cell death 4 (neoplastic transformation inhibitor) (PDCD4), transcript variant 2, matrix metalloproteinase 2 (MMP2), insulin-like growth factor binding protein 4 (IGFBP4), retinoblastoma binding protein 7 (RBBP7), insulin-like growth factor binding protein 3 (IGFBP3), downregulated in ovarian cancer 1 (DOC1).
CONCLUSION
The result of this analysis supports the hypothesis that the endometrial cancer tissue has distinct gene expression profile from normal endometium. But, the vaildation of gene expression with RT-PCR and the further study are needed.

Keyword

Endometrial cancer; Gene expression; cDNA microarray

MeSH Terms

Apoptosis
Cell Cycle
Cell Death
Developed Countries
DNA, Complementary*
Endometrial Neoplasms*
Endometrium
Female
Fibronectins
Gene Expression*
Humans
Insulin-Like Growth Factor Binding Protein 3
Insulin-Like Growth Factor Binding Protein 4
Interleukin-1 Receptor-Associated Kinases
Matrix Metalloproteinase 2
Oligonucleotide Array Sequence Analysis*
Ovarian Neoplasms
Retinoblastoma-Binding Protein 7
Transcriptome*
Transforming Growth Factors
Zinc Fingers
DNA, Complementary
Fibronectins
Insulin-Like Growth Factor Binding Protein 3
Insulin-Like Growth Factor Binding Protein 4
Interleukin-1 Receptor-Associated Kinases
Matrix Metalloproteinase 2
Retinoblastoma-Binding Protein 7
Transforming Growth Factors