Korean J Fertil Steril.  2007 Mar;34(1):19-31.

Comparison of Gene Expression Profile in Eutopic Endometria with or without Endometriosis: A Microarray Study

• Chung MJ
• Chung EJ
• Lee SJ
• Kim MK
• Chun SS
• Lee TH

Abstract

OBJECTIVE
Pathogenesis of the endometriosis is very complex and the etiology is still unclear. Our hypothesis is that there may be some difference in gene expression patterns between eutopic endometriums with or without endometriosis. In this study, we analyzed the difference of gene expression profile with cDNA microarray.
METHODS
Endometrial tissues were gathered from patients with endometriosis or other benign gynecologic diseases. cDNA microarray technique was applied to screen the different gene expression profiles from early and late secretory phase endometria of those two groups. Each three mRNA samples isolated from early and late secretory phase of endometrial tissues of control were pooled and used as master controls and labeled with Cy3-dUTP. Then the differences of gene expression pattern were screened by comparing eutopic endometria with endometriosis, which were labeled with Cy5-dUTP. Fluorescent labeled probes were hybridized on a microarray of 4,800 human genes.
RESULTS
Twelve genes were consistently overexpressed in the endometrium of endometriosis such as ATP synthase H transporting F1 (ATP5B), eukaryotic translation elongation factor 1, isocitrate dehydrogenase 1 (NADP+), mitochondrial ribosomal protein L3, ATP synthase H+ transporting (ATP5C1) and TNF alpha factor. Eleven genes were consistently down-regulated in the endometriosis samples. Many extracellular matrix protein genes (decorin, lumican, EGF-containing fibulin-like extracellular matrix protein 1, fibulin 5, and matrix Gla protein) and protease/protease inhibitors (serine proteinase inhibitor, matrix metalloproteinase 2, tissue inhibitor of metalloproteinase 1), and insulin like growth factor II associated protein were included. Expression patterns of selected eight genes from the cDNA microarray were confirmed by quantitative RT-PCR or real time RT-PCR.
CONCLUSION
The result of this analysis supports the hypothesis that the endometrium from patients with endometriosis has distinct gene expression profile from control endometrium without endometriosis.

Keyword

Endometriosis; Pathogenesis; DNA microarray; Gene expression profile

MeSH Terms

Adenosine Triphosphate
Endometriosis*
Endometrium*
Extracellular Matrix
Female
Gene Expression*
Genital Diseases, Female
Humans
Insulin-Like Growth Factor II
Isocitrate Dehydrogenase
Matrix Metalloproteinase 2
Oligonucleotide Array Sequence Analysis
Peptide Elongation Factor 1
Ribosomal Proteins
RNA, Messenger
Transcriptome*
Adenosine Triphosphate
Insulin-Like Growth Factor II
Isocitrate Dehydrogenase
Matrix Metalloproteinase 2
Peptide Elongation Factor 1
RNA, Messenger
Ribosomal Proteins