Korean J Fertil Steril.  2005 Sep;32(3):253-260.

mRNA Expression of Thrombospondin-1 and -2 in Severe Endometriosis Patients in Korean Women

• Hur SE
• Lee JY
• Moon HS
• Chung HW

Abstract

OBJECTIVE
We investigated the expression of TSP-1 and -2 in eutopic endometrium of advanced endometriosis and control patients.
METHODS
The 33 endometriosis patients and 32 controls were enrolled. Endometrial samples were obtained from 65 premenopausal women aged 29-44 years, undergoing laparoscopic surgery or hysterectomy for non-malignant lesions. Sufficient samples were collected from 33 patients with endometriosis stage III and IV and 32 controls without endometriosis confirmed by laparoscopic surgery. The mRNA expression from eutopic endometrium for TSP-1 and -2 were analyzed by RT-QC PCR.
RESULTS
The mRNAs of TSP-1 and -2 were expressed in eutopic endometrium from endometriosis and normal controls throughout the menstrual cycle. There were no significant differences in expression of TSP-1 and TSP-2 in eutopic endometrium between controls and endometriosis patients.
CONCLUSION
Our results indicated that TSP-1 and -2 had no crucial role compared to other molecules in the regulation of angiogenesis. These findings also suggest that dysregulation of other angiogenic regulators would be concerned in pathophysiologic role in endometriosis development.

Keyword

Thrombospondin; Endometriosis; Angiogenesis

MeSH Terms

Endometriosis*
Endometrium
Female
Humans
Hysterectomy
Laparoscopy
Menstrual Cycle
Polymerase Chain Reaction
RNA, Messenger*
Thrombospondin 1
Thrombospondins
RNA, Messenger
Thrombospondin 1
Thrombospondins