Korean J Crit Care Med.  2004 Dec;19(2):106-114.

The Effect of Sevoflurane and Propofol on Expression of Inducible Nitric Oxide Synthase in Endotoxemic Rats

• Kim CH
• Park JH
• Baek SH
• Baik SW

Abstract

BACKGROUND
It is a well-known phenomenon that alveolar and peritoneal macrophages exposed to bacterial lipopolysaccharide (LPS) induce a large output of nitric oxide (NO) and an inducible nitric oxide synthase (iNOS) mRNA expression. The purpose of this study is actually how much NO production and iNOS mRAN expression are effected by anesthetics (sevoflurane and propofol) on endotoxemic rats. METHODS: To examine the production of NO in peritoneal macrophages, NO concentration were measured from the rats following 2 hours exposure to LPS and 2 hours administration of sevoflurane and propofol, respectively. Culture supernatants were collected 24 hours after exposure to LPS and anesthetics and assayed by ELISA (Enzyme Linked Immunosorbent Assay) for production of NO. The iNOS mRNA expression was measured using PCR (Polymerase Chain Reaction) techniques and autoradiography. RESULTS: In the control group, the NO concentration was measured at 2 hours after infusion of LPS to rats, and showed 12 4micrometer. After insufflations of anesthetics to experimental animals, NO concentration increased in the sevoflurane and propofol groups, 37 13 (p<0.05) and 29 12micrometer (p<0.05) respectively. The size and brightness of the iNOS mRAN bands were distinct in sevoflurane and propofol in order. CONCLUSIONS: There were no different in regard of NO production and hemodynamic changes but iNOS mRNA expression between sevoflurane and propofol group in endotoxemic rats. The mechanism is not clear, but it is related to the strong stimulating effects on the respiratory tract of inhalation anesthetics.

Keyword

Endotoxemic rat; iNOS mRAN expression; Nitric oxide; Propofol; Sepsis; Sevoflurane

MeSH Terms

Anesthetics
Anesthetics, Inhalation
Animals
Autoradiography
Enzyme-Linked Immunosorbent Assay
Hemodynamics
Insufflation
Macrophages, Peritoneal
Nitric Oxide
Nitric Oxide Synthase Type II*
Polymerase Chain Reaction
Propofol*
Rats*
Respiratory System
RNA, Messenger
Sepsis
Anesthetics
Anesthetics, Inhalation
Nitric Oxide
Nitric Oxide Synthase Type II
Propofol
RNA, Messenger