J Korean Ophthalmol Soc.  2009 Aug;50(8):1247-1253. 10.3341/jkos.2009.50.8.1247.

Effects of Oxidative Stress and Antioxidant on the Expression of Heme Oxygenase-1 in Human RPE

Affiliations
  • 1Department of Ophthalmology, Inha University School of Medicine, Incheon, Korea. hschin@inha.ac.kr

Abstract

PURPOSE
To evaluate the effects of oxidative stress and antioxidantson heme oxygenase-1 (HO-1) in cultured human retinal pigment epithelial (RPE) cells. METHODS: Cultured RPE cells were challenged with different concentrations of hydrogen peroxide (H2O2), and the HO-1 mRNA level was determined by RT-PCR after 24 hours and 48 hours of incubation independently. Additionally, the HO-1 mRNA level was measured after preincubating RPE cells with N-acetylcystein (NAC) as the antioxidant for 30 minutes and then challenging the cells with H2O2. RESULTS: The expression of the HO-1 mRNA level increased after H2O2 exposure, and this level was proportional to the increased H2O2 concentration (p<0.05). Expression of the HO-1 mRNA level decreased when the RPE cells were preincubated with NAC (p<0.05). CONCLUSIONS: In human RPE cells, the HO-1 level increased in proportion to the degree of oxidative stress and decreased with exposure to antioxidants. Expression of HO-1 may be helpful in preventing retinal disease, which occurs due to oxidative stresses such as age-related macular degeneration.

Keyword

Age related macular degeneration; Antioxidant; Heme oxygenase-1; Oxidative stress; Retinal pigment epithelium

MeSH Terms

Antioxidants
Heme
Heme Oxygenase-1
Humans
Hydrogen Peroxide
Macular Degeneration
Oxidative Stress
Retinal Diseases
Retinal Pigment Epithelium
Retinaldehyde
RNA, Messenger
Antioxidants
Heme
Heme Oxygenase-1
Hydrogen Peroxide
RNA, Messenger
Retinaldehyde
Full Text Links
  • JKOS
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2022 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr