Oral Biol Res.  2021 Sep;45(3):99-106. 10.21851/obr.45.03.202109.99.

Evaluation of superoxide dismutase 3, heme-oxygenase, and myeloperoxidase expression levels associated with oxidative stress in chronic periodontitis

• Yoon Y
• Kim H
• Lee JM

• KMID: 2521021
• DOI: http://doi.org/10.21851/obr.45.03.202109.99

Abstract

This study aimed to investigate the expression levels of antioxidative enzymes such as superoxide dismutase 3 (SOD3), heme-oxygenase (HO), and myeloperoxidase (MPOs), which are known to be associated with oxidative stress induction in chronic inflammatory conditions like periodontitis. We compared the expression levels of SOD3, HO, and MPO in the periodontal tissues of healthy individuals with those in the periodontal tissues of chronic periodontitis patients. The expression levels of SOD3, HO, and MPO were determined by Western blot technique, and the data were statistically analyzed. The expression levels of SOD3 and HO increased in periodontitis patients compared with those in healthy controls, and the degree of increase was dependent on the severity of periodontitis (p<0.05). In contrast, the expression level of MPO was lower in the periodontal tissues of healthy controls than that in the periodontal tissues of periodontitis patients (p<0.05). SOD and HO are involved in oxidative stress via antioxidative activity, and MPO is involved in oxidative stress via peroxidative activity. Hence, these findings suggest that antioxidative activity depends on the severity of inflammation. In conclusion, SOD3, HO, and MPO may play a role in oxidative stress-induced periodontitis, and these three factors may be related to the severity of inflammation. These factors are expected to be applied to the diagnosis and treatment of periodontitis through more comprehensive studies.

Keyword

Chronic periodontitis; Heme oxygenase; Myeloperoxidase; Oxidative stress; Superoxide dismutase