J Korean Acad Periodontol.  2007 Sep;37(3):553-562. 10.5051/jkape.2007.37.3.553.

Induction of osteoclastogenesis-inducing cytokines and invasion by alive Aggregatibacter actinomycetemcomitans in osteoblasts

• Choi HK
• Lee YS
• Kim MY
• Kim KD
• Cha JH
• Yoo YJ

• KMID: 2191722
• DOI: http://doi.org/10.5051/jkape.2007.37.3.553

Abstract

Osteoblasts regulate osteoclastogenesis by production of various cytokines. Aggregatibacter(A) actinomycetemcomitans is one of periodontopathogens which invades gingival tissue. Therefore, clarifying the effect of alive A. actinomycetemcomitans on osteoblasts is important to understand the mechanism of alveolar bone resorption in periodontitis. We investigated induction of osteoclastogenesis- inducing cytokines, adherence, and invasion by A. actinomycetemcomitans in osteoblasts. Osteoblasts were isolated from mouse calvaria and expression of cytokines was determined by RT-PCR. When the ratio of the number of A. actinomycetemcomtians to the number of osteoblasts was 10:1, 50:1 and 100:1, RANKL mRNA expression was increased. A. actinomycetemcomitans also increased expression of macrophage inflammatory protein (MIP)-1alpha, interleukin (IL)-1beta, and tumor necrosis factor (TNF)-alpha. A. actinomycetemcomitans attached to and invaded osteoblasts at ratio of 1000:1. These results suggest that A. actinomycetemcomitans increases osteoclastogenesis-inducing ability of osteoblasts by stimulating the expression of RANKL, MIP-1alpha, IL-1beta, and TNF-alpha and that invasion of A. actinomycetemcomitans provides a means by which the bacteria escape from immune system and antibiotic therapy.

Keyword

Aggregatibacter actinomycetemcomitans; osteoblast; osteoclastogenesis-inducing cytokine; invasion

MeSH Terms

Aggregatibacter actinomycetemcomitans*
Aggregatibacter*
Animals
Bacteria
Bone Resorption
Chemokine CCL3
Cytokines*
Immune System
Interleukins
Macrophages
Mice
Osteoblasts*
Periodontitis
RNA, Messenger
Skull
Tumor Necrosis Factor-alpha
United Nations
Chemokine CCL3
Cytokines
Interleukins
RNA, Messenger
Tumor Necrosis Factor-alpha