J Korean Med Assoc.  2009 Oct;52(10):1007-1019. 10.5124/jkma.2009.52.10.1007.

Mitochondrial Permeability Transition Pore and Cardioprotection Against Ischemia-reperfusion Injury

• Lee JW
• Shim YH

• KMID: 2188082
• DOI: http://doi.org/10.5124/jkma.2009.52.10.1007

Abstract

Opening of mitochondrial permeability transition pore (mPTP) was found to have a critical role in cell death from ischemia/reperfusion (I/R) injury experimentally in the late 1980's. Thereafter, tremendous efforts have been made to define the molecular composition of mPTP and underlying mechanisms of its opening. mPTP opening, so far, has been demonstrated with the conformational changes of the mitochondrial protein components including cyclophilin-D, adenine nucleotide translocase, and voltage-dependent anion channel, which were induced by the modification of the levels of Ca2+, phosphate, mitochondrial membrane potential, intracellular pH and adenine nucleotide. At present, genetic modulation of the expression of protein components are being used in the investigation of its properties, presenting novel mechanisms of mPTP opening, including phosphate carrier. For therapeutic intervention, cyclosporin A and its analogues were first to be demonstrated to inhibit the opening of mPTP, affecting cyclophilin-D. There are numerous pharmacological substances that have direct or indirect effects on mPTP opening, including bongkrekic acid, reactive oxygen species scavengers, calcium channel blockers, and Na+/H+ exchanger-1 inhibitors, but only cyclosporin A was clinically tried to limit the myocardial infarction. Conditioning interventions, ischemic or anesthetic, have also been shown to be effective in limiting the detrimental effects of I/R injury. These interventions are commonly related to specific receptors on cell membrane and then signal transduction pathway consisting of many protein kinases, which eventually lead to mitochondria. And being presented are experimental evidences that inhibition of mPTP opening is a primary mechanism of these conditioning interventions. In conclusion, mPTP opening is now presented as primary mechanism and therapeutic target of I/R injury, but precise mechanism and standardized treatment method are needed to be clarified.

Keyword

Mitochondrial permeability transition pore; Cardioprotection; Ischemia/reperfusion injury; Myocardium

MeSH Terms

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Adenine
Bongkrekic Acid
Calcium Channel Blockers
Cell Death
Cell Membrane
Cyclosporine
Hydrogen-Ion Concentration
Membrane Potential, Mitochondrial
Mitochondria
Mitochondrial ADP, ATP Translocases
Mitochondrial Membrane Transport Proteins
Mitochondrial Proteins
Myocardial Infarction
Myocardium
Permeability
Protein Kinases
Reactive Oxygen Species
Reperfusion Injury
Signal Transduction
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Adenine
Bongkrekic Acid
Calcium Channel Blockers
Cyclosporine
Mitochondrial ADP, ATP Translocases
Mitochondrial Membrane Transport Proteins
Mitochondrial Proteins
Protein Kinases
Reactive Oxygen Species

Figure

• 

  Figure 1 Molecular models for the mitochondrial permeability transition pore (mPTP). (Left) The original model for the mPTP, of the voltage-dependent anion channel (VDAC) in the outer mitochondrial membrane (OMM), the adenine nucleotide translocase (ANT) in the inner mitochondrial membrane (IMM), and cyclophilin-D (Cyp-D) in the matrix. (Right) Revised models in light of recent findings in gene-targeted mice. VDAC is no longer part of the model and it appears that an outer membrane component may not even be necessary for this process. ANT now appears to be more of a regulatory protein, and only Cyp-D remains as an established component. In contrast, the mitochondrial phosphate carrier (PiC) has been added to model as a potential candidate for the pore-forming unit of the mPTP. Figure is taken from reference (10).

• 

  Figure 2 Scheme to illustrate the involvement of mPTP opening in reperfusion injury and how various cardioprotective agents may prevent this. ⤑ represent changes that occur primarily in ischemia → depict changes that occur primarily during reperfusion whilst dotted ➡ indicate the locus of action of cardioprotective agents Figure is taken from reference (44).

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