Biomol Ther.  2016 Jan;24(1):49-56. 10.4062/biomolther.2015.056.

Aloe-Emodin Protects RIN-5F (Pancreatic beta-cell) Cell from Glucotoxicity via Regulation of Pro-Inflammatory Cytokine and Downregulation of Bax and Caspase 3

• Alshatwi A
• Subash-Babu P

• KMID: 2170540
• DOI: http://doi.org/10.4062/biomolther.2015.056

Abstract

To determine the protective effect of aloe-emodin (AE) from high glucose induced toxicity in RIN-5F (pancreatic beta-cell) cell and restoration of its function was analyzed. RIN-5F cells have been cultured in high glucose (25 mM glucose) condition, with and without AE treatment. RIN-5F cells cultured in high glucose decreased cell viability and increased ROS levels after 48 hr compared with standard medium (5.5 mM glucose). Glucotoxicity was confirmed by significantly increased ROS production, increased pro-inflammatory (IFN-gamma, IL-1beta,) & decreased anti-inflammatory (IL-6&IL-10) cytokine levels, increased DNA fragmentation. In addition, we found increased Bax, caspase 3, Fadd, and Fas and significantly reduced Bcl-2 expression after 48 hr. RIN-5F treated with both high glucose and AE (20 muM) decreased ROS generation and prevent RIN-5F cell from glucotoxicity. In addition, AE treated cells cultured in high glucose were transferred to standard medium, normal responsiveness to glucose was restored within 8hr and normal basal insulin release within 24 hr was achieved when compared to high glucose.

Keyword

Aloe-emodin; Glucotoxicity; RIN-5F cells; Apoptosis; ROS

MeSH Terms

Apoptosis
Caspase 3*
Cell Survival
DNA Fragmentation
Down-Regulation*
Glucose
Insulin
Caspase 3
Glucose
Insulin