Endocrinol Metab.  2015 Dec;30(4):569-575. 10.3803/EnM.2015.30.4.569.

Omega-3 Polyunsaturated Fatty Acids May Attenuate Streptozotocin-Induced Pancreatic beta-Cell Death via Autophagy Activation in Fat1 Transgenic Mice

Affiliations
  • 1Division of Nephrology, Department of Internal Medicine, Konyang University College of Medicine, Daejeon, Korea.
  • 2Myunggok Research Institute, Konyang University College of Medicine, Daejeon, Korea. mdldm@hanmail.net
  • 3Department of Anatomy, Konyang University College of Medicine, Daejeon, Korea.
  • 4Division of Endocrinology, Department of Internal Medicine, Konyang University College of Medicine, Daejeon, Korea.
  • 5Department of Biochemistry, Chungnam National University School of Medicine, Daejeon, Korea.
  • 6Cancer Research Institute, Chungnam National University School of Medicine, Daejeon, Korea.

Abstract

BACKGROUND
Inflammatory factors and beta-cell dysfunction due to high-fat diets aggravate chronic diseases and their complications. However, omega-3 dietary fats have anti-inflammatory effects, and the involvement of autophagy in the etiology of diabetes has been reported. Therefore, we examined the protective effects of autophagy on diabetes using fat-1 transgenic mice with omega-3 self-synthesis capability.
METHODS
Streptozotocin (STZ) administration induced beta-cell dysfunction in mice; blood glucose levels and water consumption were subsequently measured. Using hematoxylin and eosin (H&E) and Masson's trichrome staining, we quantitatively assessed STZ-induced changes in the number, mass, and fibrosis of pancreatic islets in fat-1 and control mice. We identified the microtubule-associated protein 1A/1B light chain 3-immunoreactive puncta in beta-cells and quantified p62 levels in the pancreas of fat-1 and control mice.
RESULTS
STZ-induced diabetic phenotypes, including hyperglycemia and polydipsia, were attenuated in fat-1 mice. Histological determination using H&E and Masson's trichrome staining revealed the protective effects of the fat-1 expression on cell death and the scarring of pancreatic islets after STZ injection. In the beta-cells of control mice, autophagy was abruptly activated after STZ treatment. Basal autophagy levels were elevated in fat-1 mice beta-cells, and this persisted after STZ treatment. Together with autophagosome detection, these results revealed that n-3 polyunsaturated fatty acid (PUFA) enrichment might partly prevent the STZ-related pancreatic islet damage by upregulating the basal activity of autophagy and improving autophagic flux disturbance.
CONCLUSION
Fat-1 transgenic mice with a n-3 PUFA self-synthesis capability exert protective effects against STZ-induced beta-cell death by activating autophagy in beta-cells.

Keyword

Omega 3 fatty; Beta cell; Fat-1 transgenic mice
Full Text Links
  • ENM
Share
  • Twitter
  • Facebook
Copyright © 2020 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr