J Biomed Res.  2015 Sep;16(3):98-103. 10.12729/jbr.2015.16.3.098.

Uterine expression of tight junctions in the Canine uterus

Affiliations
  • 1Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Korea. ebjeung@chungbuk.ac.kr

Abstract

Tight junctions (TJs) form continuous intercellular contacts in intercellular junctions. TJs involve integral proteins such as occludin (OCLN) and claudins (CLDNs) as well as peripheral proteins such as zona occludens-1 (ZO-1) and junctional adhesion molecules (JAMs). TJs control paracellular transportation across cell-to-cell junctions. Although TJs have been studied for several decades, comparison of the transcriptional-translational levels of these molecules in canine organs has not yet been performed. In this study, we examined uterine expression of CLDNs, OCLN, junction adhesion molecule-A, and ZO-1 in canine. Expression levels of canine uterine TJ proteins, including CLDN1, 2, 4, 5, JAM-A, ZO-1, and OCLN, were measured using reverse transcription PCR, real-time PCR, and Western blotting, whereas TJs distribution was determined by immunohistochemistry. The mRNA and protein expression levels of OCLN, CLDN-1, 4, JAM-1, and ZO-1 were identified in the uterus. Immunohistochemistry demonstrated that TJs were localized to the endometrium and/or myometrium of the uterus. Our results show that canine TJ proteins, including CLDNs, OCLN, JAM-A, and ZO-1, were expressed in the canine uterus. Taken together, these proteins may perform unique physiological roles in the uterus. Therefore, these findings may serve as a basis for further studies on TJ proteins and their roles in the physiological or pathological condition of the canine uterus.

Keyword

physiology; intercellular Junctions; Tight Junctions; uterus; dogs

MeSH Terms

Animals
Blotting, Western
Claudins
Dogs
Endometrium
Female
Herpes Zoster
Immunohistochemistry
Intercellular Junctions
Junctional Adhesion Molecules
Mice
Myometrium
Occludin
Physiology
Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
Reverse Transcription
RNA, Messenger
Tight Junctions*
Transportation
Uterus*
Claudins
Junctional Adhesion Molecules
Occludin
RNA, Messenger
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