Cancer Res Treat.
2001 Jun;33(3):236-242.
Growth Suppression of Ovarian Cancer Cells by Interferon-gama
- Affiliations
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- 1Department of Obstetrics and Gynecology, The Catholic University Medical College.
- 2Department of Anatomy, The Catholic University Medical College.
- 3Catholic Cancer Research Institute, Catholic Research Institute of Medical Science, Seoul, Korea.
- 4Department of Life Science, Sejong University, Seoul, Korea.
Abstract
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PURPOSE: Growth regulation of cancer cells very frequently involves tumor suppressor gene p53, Rb and cell cycle
regulator, however the molecular biologic mechanisms of growth regulation in ovarian carcinoma cells are not fully
defined. To assess the mechanism of growth suppression, we treated IFN-gama in ovarian carcinoma cells.
MATERIALS AND METHODS
Growth suppression by treatment of IFN-gama was determined by cell proliferation assay in ovarian carcinoma cell lines. Apoptosis was determined by DNA fragmentation assay and electron microscopy. Molecular mechanism of the apoptosis in ovarian carcinoma cell by IFN-gama was further analyzed by the western blot.
RESULTS
We found that IFN-gama had remarkable growth- suppressive effects in PA-1 and A2774 ovarian carcinoma cells in a time-dependent manner. Apoptosis was observed in PA-1 and A2774 cell following treatment of IFN- gama by DNA fragmentation assay and EM. The expression of IRF-1 protein from A2774 and PA-1 cell extracts was elevated by increasing the concentration of IFN-gama. IFN-gama caused an increased expression of the important apoptosis-related gene, ICE (interleukin-1beta-converting enzyme) protein in A2774 and PA-1.
CONCLUSION
The coordinate induction of IRF-1 and ICE by IFN-gama in ovarian carcinoma cells suggests a functional relationship between these proteins in programmed cell death. The significance of this study is the molecular biologic background of IFN-gama considered as an alternative treatment trial of ovarian cancers.
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