Cancer Res Treat.  2006 Dec;38(4):234-239.

Mouse Orthotopic Lung Cancer Model Induced by PC14PE6

• Cui ZY
• Ahn JS
• Lee JY
• Kim WS
• Lim HY
• Jeon HJ
• Suh SW
• Kim JH
• Kong WH
• Kang JM
• Nam DH
• Park K

Abstract

PURPOSE
This study was undertaken to investigate in detail the xenograft mouse orthotopic lung cancer model induced by PC14PE6 adenocarcinoma cells.
MATERIALS AND METHODS
Three cell doses (0.5x10(6); 1x10(6); 2x10(6)) of PC14PE6 cells were injected into the lungs of male BALB/c nude mice by the intrathoracic injection method. The lung and other organs, including brain, liver, spleen, kidney, muscle, adrenal gland, and lymph node on knee, were removed and stained with H/E to detect the presence of tumor cells.
RESULTS
The reliable tumorigenicity time in the PC14PE6 adenocarcinoma cell-inoculated BALB/c nude mouse was 10 days after intrathoracic injection. The average life span of the three groups after inoculation was 14 days in the 2x10(6) cells inoculum group; 25 days in the 1x10(6) cells inoculum group; and 32 days in the 0.5x10(6) cells inoculum group. The PC14PE6 adenocarcinoma cells induced orthotopic lung cancer limited within the thorax.
CONCLUSIONS
This orthotopic lung cancer model is an efficient cancer model with easy inoculation methods, rapid and high tumorigenicity, and simple monitoring methods for metastasis.

Keyword

Orthotopic; PC14PE6; Lung cancer; Animal model

MeSH Terms

Adenocarcinoma
Adrenal Glands
Animals
Brain
Heterografts
Humans
Kidney
Knee
Liver
Lung Neoplasms*
Lung*
Lymph Nodes
Male
Mice*
Mice, Nude
Models, Animal
Neoplasm Metastasis
Spleen
Thorax

Figure

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  Fig. 1 Microscopic features of the mouse lung. H/E stain of paraffin-embedded sections of tumors within the mouse lung. The solid tumor mass (blue) was seen in the right image contrasting to the left normal one (magnification ×100).

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  Fig. 2 Microscopic features of the chest lymph node. H/E staining images of the paraffin-embedded sections of tumor developed within mouse lymph node in the chest. The center of the lymph node was occupied by PC14PE6 cancer cells (right) in contrast to the normal lymph node (left, magnification ×200).

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  Fig. 3 Average life span of three groups in the preliminary experiment. The mice were inoculated by 0.5×106; 1×106; and 2×106 PC14PE6 lung adenocarcinoma cells.

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  Fig. 4 Macroscopic features of the mouse thoracic cavity. PC14PE6 cells were injected into the thorax of nude mice, and experimental lung cancer was determined every 3 days beginning 4 days after inoculation. The tumor mass developed rapidly in the PC14PE6 cancer cell-inoculated nude mice.

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  Fig. 5 Microscopic features of the mouse lung. H/E stain of the paraffin-embedded sections of tumor developed within mouse lung. The solid tumor mass was seen in the PC14PE6 cancer cell-inoculated nude mice 10 days after inoculation and rapidly developed in the lung day by day (magnification ×40).

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