Maxillofac Plast Reconstr Surg.  2016 Mar;38(3):17. 10.1186/s40902-016-0062-4.

Cell attachment and proliferation of osteoblast-like MG63 cells on silk fibroin membrane for guided bone regeneration

• Yoo CK
• Jeon JY
• Kim YJ
• Kim SG
• Hwang KG

• KMID: 2161831
• DOI: http://doi.org/10.1186/s40902-016-0062-4

Abstract

BACKGROUND
The aim of this study is to verify the feasibility of using silk fibroin (SF) as a potential membrane for guided bone regeneration (GBR).
METHODS
Various cellular responses (i.e., cell attachment, viability, and proliferation) of osteoblast-like MG63 cells cultured on an SF membrane were quantified. After culturing on an SF membrane for 1, 5, and 7 days, the attachment and surface morphology of MG63 cells were examined by optical and scanning electron microscopy (SEM), cell viability was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell proliferation was quantified using 4',6-diamidino-2-phenylindole (DAPI) fluorescence staining.
RESULTS
Optical microscopy revealed that MG63 cells cultured on the SF membrane proliferated over the 7-day observation period. The viability of cells cultured on SF membranes (SF group) and on control surfaces (control group) increased over time (P < 0.05); however, at respective time points, cell viability was not significantly different between the two groups (P > 0.05). In contrast, cell proliferation was significantly higher in the SF membrane group than in the control group at 7 days (P < 0.05).
CONCLUSIONS
These results suggest that silk fibroin is a biocompatible material that could be used as a suitable alternative barrier membrane for GBR.

Keyword

Bone regeneration; Cell adhesion; Cell proliferation; Membrane; Osteoblasts; Silk fibroin

MeSH Terms

Bone Regeneration*
Cell Adhesion
Cell Proliferation
Cell Survival
Fibroins*
Fluorescence
Membranes*
Microscopy
Microscopy, Electron, Scanning
Osteoblasts
Silk*
Fibroins
Silk