Tuberc Respir Dis.  2000 Jun;48(6):879-886. 10.4046/trd.2000.48.6.879.

DNAse I hypersensitive sites of lung specific transcription factor gene

• Lee YC

• KMID: 2160239
• DOI: http://doi.org/10.4046/trd.2000.48.6.879

Abstract

BACKGROUND
Thyroid Transcription Factor-1(TTF-1) acts as a tissue specific transcription factor in the regulation of lung specific gene expression and as morphogenic protein during lung organogenesis. Currently, there is very little information on the cis-acting sequences and transcription and transcription factors that direct the TTF-1 gene expression. DNAse 1 hypersensitive (DH) sites represent a marker for active or potentially active chromatin and are likely to be especially important in gene regulation, being associated with many DNA sequences that regulate gene expression. It is clear that DH regions correlate with genetic regulatory loci and binding for sequence-specific DNA-binding proteins.
METHODS
We have used DH site assays to identify putative distal regulatory elements in H441 lung adenocarcinoma cells, which express the TTF-1 gene and HeLa cells.
RESULTS
There are four DH sites 5' of the TTF-1 gene. These sites are located at base pair approximately +150, -450, -800, and -1500 from the start of transcription.
CONCLUSION
These data suggest that there may be at least one intragenic site and regulatory region 5' prime to the promotor region.

Keyword

Transcription; Lung; Gene

MeSH Terms

Adenocarcinoma
Base Pairing
Base Sequence
Chromatin
Deoxyribonuclease I*
Deoxyribonucleases*
DNA-Binding Proteins
Gene Expression
HeLa Cells
Humans
Lung*
Organogenesis
Promoter Regions, Genetic
Regulatory Sequences, Nucleic Acid
Thyroid Gland
Transcription Factors*
Chromatin
DNA-Binding Proteins
Deoxyribonuclease I
Deoxyribonucleases
Transcription Factors