J Korean Soc Microbiol.  2000 Jun;35(3):239-250.

Antimicrobial resistance and molecular epidemiologic characteristics of Stenotrophomonas maltophilia isolated from clinical specimens

• Seol SY
• Jang KS
• Jeong OG
• Cho ER
• Kim NH
• Yu HS
• Lee YC
• Cho DT

Abstract

Sixty-eight clinical isolates of Stenotrophomonas maltophilia from inpatients of 2 university hospitals in Taegu were epidemiologically analyzed by using the minimum inhibitory concentrations of 25 antimicrobial drugs, biochemical reaction, pulsed-field gel elctropgoresis (PFGE), and PCR with enterobacterial repetitive intergenic consensus sequences as primer (ERIC-PCR). 1. All the strains were susceptible to minocycline. More than 57% were susceptible to sulfisomidine (Su), ciprofloxacin (Ci), Ofloploxacin (Of), nalidixic acid (Na), and chloramphenicol (Cm), and 19apprx35% to ceftazidime (Cd), trimethoprim (Tp), Ticacillin-clavulanic acid, and cefoperazone-sulbactam. Most isolates were resistant to beta-lactam antibiotics such as ampicillin (Ap), carbenicillin (Cb), cefotaxim (Ct), cefoxitin (Cx), and aminoglycosides including gentamicin (Gm), tobramycin (Tb), amikacin (Ak). 2. All the isolates were multiply resistant of 5 to 17 drugs and showed 40 different resistance pattern types. 3. All the strains showed very similar biochemical reactions except beta-galactosidase and nitrate reduction test. Fourteen strains selected randomly were classified 10 different pattern type by PFGE and ERIC-PCR. These two methods showed identical result. Four strains isolated from wound in 1994 showed similar MIC pattern and identical API 20NE profile, PFGE, and ERIC-PCR pattern indicating episodes of cross-infection among patients. These results indicate that PFGE or ERIC-PCR profile has comparable discriminatory power for epidemiological typing of S. maltophilia.

MeSH Terms

Amikacin
Aminoglycosides
Ampicillin
Anti-Bacterial Agents
beta-Galactosidase
Carbenicillin
Cefotaxime
Cefoxitin
Ceftazidime
Chloramphenicol
Ciprofloxacin
Consensus Sequence
Daegu
Gentamicins
Hospitals, University
Humans
Inpatients
Microbial Sensitivity Tests
Minocycline
Nalidixic Acid
Polymerase Chain Reaction
Stenotrophomonas maltophilia*
Stenotrophomonas*
Sulfisomidine
Tobramycin
Trimethoprim
Wounds and Injuries
Amikacin
Aminoglycosides
Ampicillin
Anti-Bacterial Agents
Carbenicillin
Cefotaxime
Cefoxitin
Ceftazidime
Chloramphenicol
Ciprofloxacin
Gentamicins
Minocycline
Nalidixic Acid
Sulfisomidine
Tobramycin
Trimethoprim
beta-Galactosidase