Korean J Prev Med.
2001 Feb;34(1):41-46.
Effects of Particulate Matters on A549 and RAW 264.7 Cells
- Affiliations
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- 1Department of Occupational and Environmental Medicine, St. Mary's Hospital, The Catholic University of Korea, Seoul, Korea.
Abstract
OBJECTIVES
To investigate the effects of particulate matter (PM), a marker of environmental pollution derived from combustion sources, on lung epithelial cells (A549) and macrophage (RAW 264.7).
METHODS
The production of reactive radicals from lung cells, the lipid peroxidation of cell membrane, and the cytotoxicity of PM were measured using an in vitro model. The
results
were compared with a control group.
RESULTS
The presence of PM significantly increased the production of reactive oxygen species and reactive nitrogen species with time and in a dose dependent pattern and also increased the malondialdehyde concentration in lung epithelial cells. The cytotoxicity of PM was increased with increasing concentration of PM.
CONCLUSIONS
It has been suggested that urban particulate matter causes an inflammatory reaction in lung tissue through the production of hydroxyl radicals, nitric oxides and numerous cytokines. The causal chemical determinant responsible for these biologic effects are not well understood, but the bioavailable metal in PM seems to determine the toxicity of inhaled PM.
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