Mycobiology.  2006 Sep;34(3):143-147. 10.4489/MYCO.2006.34.3.143.

Mycelial Extract of Phellinus linteus Induces Cell Death in A549 Lung Cancer Cells and Elevation of Nitric Oxide in Raw 264.7 Macrophage Cells

• Lee JJ
• Kwon HK
• Lee DS
• Lee SW
• Lee KK
• Kim KJ
• Kim JL

• KMID: 1598822
• DOI: http://doi.org/10.4489/MYCO.2006.34.3.143

Abstract

In the present study, in order to investigate the anti-proliferative phenomenon of PLME, the effects of mycelial extract of Phellinus linteus (PLME) on the growth of human lung carcinoma cell line A549 was examined. We studied on the effects of PLME on the release of nitric oxide (NO) in mouse macrophage Raw 264.7 cells. Treatment of PLME to A549 cells resulted in the growth inhibition, morphological change and induction of apoptotic cell death in a dose-dependent manner as measured by MTT assay. We found that PLME stimulated a dose-dependent increase in NO production. These findings suggest that PLME enhances the anti-tumoral activity of macrophage and may be a potential therapeutic agent for the control of human lung carcinoma cells.

Keyword

A549; Nitric oxide; Phellinus linteus; PLME; Raw 264.7

MeSH Terms

Animals
Cell Death*
Cell Line
Humans
Lung Neoplasms*
Lung*
Macrophages*
Mice
Nitric Oxide*
Nitric Oxide