J Korean Ophthalmol Soc.  2002 Nov;43(11):2288-2299.

The effect of Hydrogen Peroxide and Ascorbic Acid on Proliferation and Morphology of Lens Epithelial Cells

• Lee JH
• Oh JH
• Cho JG
• Kang SJ

Abstract

PURPOSE: The therapeutic effects of amniotic membrane transplantation (AMT) contain anti-fibrosis, anti-inflammation and facilitation of epithelialization. Thus we investigated whether applying of amniotic membrane (AM) ointment could get the same effect as AMT.
METHODS
Alkali burn was induced by applying 6 mm round filter paper which was soaked with 1 N NaOH, onto the central cornea for 60 seconds on both eyes of 10 white rabbits. Then we applied AM ointment on one eye and base ointment on the other eye, four times a day for 1 week. The corneas were harvested for histopathologic studies at 1 day and 3 weeks. Corneal opacity and thickness were measured in 3 days and 1, 2, 3 weeks.
RESULTS
In the AM ointment applicated group, the number of the infiltrated PMNs (polymorp-honuclear cells) and apoptotic keratocytes were significantly less than that of the control group (P<0.05). The degree of lipid peroxidation and myofibroblast differentiation were less than those of the control group. Corneal opacity and corneal edema were less in AM ointment group than control group.
CONCLUSIONS
AM ointment application after alkali burn is beneficial to reduce inflammation, keratocyte apoptosis and lipid peroxidation, and is considered to suppress corneal haze by these effects. Therefore, this report may be a basic study for the AM ointment research to treat recalcitrant keratitis.

Keyword

Ascorbic acid; Cataract; Fibronectin; Hydrogen peroxide; Lens epithelial cell

MeSH Terms

Alkalies
Amnion
Apoptosis
Ascorbic Acid*
Burns
Cataract
Cornea
Corneal Edema
Corneal Opacity
Epithelial Cells*
Fibronectins
Hydrogen Peroxide*
Hydrogen*
Inflammation
Keratitis
Lipid Peroxidation
Myofibroblasts
Rabbits
Alkalies
Ascorbic Acid
Fibronectins
Hydrogen
Hydrogen Peroxide