Korean J Hematol.
2000 May;35(2):97-108.
Establishment of Multi-Drug Resistance Functional Assay and Correlation of Multi-Drug Resistance Activity and Chemotherapeutic Outcomes in Patients with Acute Leukemia
- Affiliations
-
- 1Department of Clinical Pathology, Asan Medical Center, Ulsan University College of Medicine, Seoul, Korea.
- 2Department of Internal Medicine, Asan Medical Center, Ulsan University College of Medicine, Seoul, Korea.
Abstract
-
BACKGROUND: Multi-drug resistance (MDR) is one of the most important obstacles in the chemotherapy of acute leukemia, so the modulators of MDR have been developed and tried.
METHODS
We measured MDR function and expressoin (surface and cytoplasmic p-glycoprotein and cytoplasmic multidrug-resistance associated protein (MRP)) and inhibitory effects of MDR modulators (cyclosporine and verapamil) by flow cytometry with MDR positive cell line and bone marrow aspirates of patients with acute leukemia (128 specimen). We compared these methods, and tried to clarify the effects of MDR on chemotherapy in patients with acute leukemia.
RESULTS
The MDR functional assay and the detection method for inhibitory effects of MDR modulators (cyclosporine and verapamil) by flow cytometry using rhodamine 123 were established. These MDR functional assay was more sensitive, accurate, relatively simple and very economic, compared with the immunofluorescence assays of surface and cytopla-smic p-glycoprotein and cytoplasmic MRP. The positivity of MDR functional assay was observed in about 60% of patients with acute leukemia, and MDR activity (%) was inversely correlated with the complete remission rate and mean survival time. About 60% of the patients showing positive MDR activity revealed MDR inhibitory responses by cyclosporine and/or verapamil, especially all cases of acute myeloid leukemia in persistence after chemotherapy showed MDR inhibitory effect of cyclosporine.
CONCLUSION
The chemotherapeutic out- comes of acute leukemia can be expected by MDR functional assay. And it is possible to overcome MDR by the administration of MDR modulator selected according to the results of the functional assay for MDR inhibitory effect in acute leukemia patients with MDR positivity.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Multi-drug resistance (MDR1) gene expression in de novo acute leukemia cells: correlations with CD surface markers and treatment outcome
- A role of Cyclosporine A that suppresses multi-drug resistance (MDR) in the secondary chemotherapy drug resistant cell line of ovarian cancer
- Mechanical Alteration Associated With Chemotherapeutic Resistance of Breast Cancer Cells
- Relation among the Tests and Comparison of Positivity of Tests for Multi-Drug Resistance in Newly Diagnosed Acute Leukemia
- Significance of Multidrug Resistance (MDR) Activity in Remission Induction & Survival of Acute Myeloid Leukemia
