Gut Liver.  2014 Sep;8(5):487-494. 10.5009/gnl13133.

RING Finger Proteins Are Involved in the Progression of Barrett Esophagus to Esophageal Adenocarcinoma: A Preliminary Study

• Wang XW
• Wei W
• Wang WQ
• Zhao XY
• Guo H
• Fang DC

• KMID: 2070058
• DOI: http://doi.org/10.5009/gnl13133

Abstract

BACKGROUND/AIMS
To investigate the differential expression of RING finger (RNF) proteins in Barrett esophagus (BE) and esophageal adenocarcinoma (EAC).
METHODS
The differential expression of RNFs in normal esophagus (NE), BE, and EAC was screened using microarray assay. Real-time quantitative polymerase chain reaction (PCR), tissue microarray assay, and Western blot analysis were independently performed to detect the mRNA and protein expression of screened RNFs.
RESULTS
The expression of nine RNFs in the BE or EAC was 2-fold higher than those in NE. Among these proteins, the RNF32 and RNF121 expression in BE was 20.3-fold and 16.4-fold higher, respectively, than that in NE, and the expression of RNF24, RNF130, RNF141, RNF139, RNF11, RNF14, and RNF159 was upregulated more than 2-fold compared with NE. The expression of nine RNFs was not only upregulated in the EAC but was also positively related to the RNF expression in BE. The PCR results also indicated increased expression of these RNFs in BE and EAC compared to NE. Furthermore, the mRNA expression of all RNFs, except for RNF141 in EAC, was dramatically higher than those in the BE. Similar results were also obtained from the Western blot analysis.
CONCLUSIONS
A total of nine RNFs play critical roles in the progression of BE to EAC.

Keyword

Barrett esophagus; Esophageal adenocarcinoma; RING finger proteins; Tissue array analysis

MeSH Terms

Adenocarcinoma/*enzymology/genetics
Adult
Aged
Barrett Esophagus/*enzymology/genetics
Carrier Proteins/genetics
Disease Progression
Esophageal Neoplasms/*enzymology/genetics
Female
Gene Expression Profiling
Humans
Intracellular Signaling Peptides and Proteins/genetics
Male
Middle Aged
Proteins/genetics
*RING Finger Domains
Receptors, Cell Surface/genetics
Ubiquitin-Protein Ligases/genetics/*metabolism
Carrier Proteins
Intracellular Signaling Peptides and Proteins
Proteins
Receptors, Cell Surface
Ubiquitin-Protein Ligases