Korean J Vet Res.  2015 Sep;55(3):199-204. 10.14405/kjvr.2015.55.3.199.

Anti-inflammatory effects of 4,4'-diaminodiphenyl sulfone (dapsone) in lipopolysaccharide-treated spleen cells: selective inhibition of inflammation-related cytokines

• Moon SY
• Joo HG

• KMID: 2049856
• DOI: http://doi.org/10.14405/kjvr.2015.55.3.199

Abstract

4,4'-diaminodiphenyl sulfone (dapsone) is a sulfone drug that has antibacterial effects on a variety of bacteria, especially Mycobacterium leprae; thus, it has been used to treat leprosy. Previous studies demonstrated that dapsone inhibits integrin-mediated adherence of neutrophils and production of prostaglandin E2 by polymorphonuclear leukocytes. Hence, dapsone may act in immune cells and regulate cell-mediated inflammation processes. However, its anti-inflammatory effects remain unclear. The present study demonstrated that dapsone modulates the production of inflammation-related cytokines in immune cells. We employed the spleen cells of mice, which are major immune cells, and lipopolysaccharide (LPS) as a causative agent of inflammation for experiments. Dapsone induced a proportional change in splenocyte subsets and the apoptosis of spleen cells. Interestingly, dapsone decreased the production of tumor necrosis factor-alpha and interleukin (IL)-10, but not IL-6, in LPS-treated spleen cells. In other assays, we measured the dapsone-induced production of nitric oxide (NO) and the expression of activation markers of spleen cells. Dapsone decreased NO production in LPS-treated spleen cells. Taken together, our results demonstrate that dapsone has anti-inflammatory effects in immune cells and provide new insight into the potential uses of this agent.

Keyword

anti-inflammatory activity; dapsone; inflammatory cytokines; spleen cells

MeSH Terms

Animals
Apoptosis
Bacteria
Cytokines*
Dapsone
Dinoprostone
Inflammation
Interleukin-6
Interleukins
Leprosy
Mice
Mycobacterium leprae
Neutrophils
Nitric Oxide
Spleen*
Tumor Necrosis Factor-alpha
Cytokines
Dapsone
Dinoprostone
Interleukin-6
Interleukins
Nitric Oxide
Tumor Necrosis Factor-alpha