Korean J Vet Res.  2019 Jun;59(2):75-80. 10.14405/kjvr.2019.59.2.75.

Modulatory action of enrofloxacin in lipopolysaccharide-induced hyper-activated mouse spleen cells

• Ko HJ
• Joo HG

• KMID: 2451641
• DOI: http://doi.org/10.14405/kjvr.2019.59.2.75

Abstract

Enrofloxacin, a fluoroquinolone, is a broad-spectrum antibiotic widely used in veterinary medicine that inhibits the action of bacterial DNA gyrase, resulting in anti-bacterial effects. This study was performed to examine whether enrofloxacin has modulatory and anti-inflammatory activity on immune cells. A few studies have reported the anti-inflammatory effects of enrofloxacin. In this study, we used mouse spleen cells treated with lipopolysaccharide (LPS) and examined the effects of enrofloxacin. Several assays were performed in LPS-treated spleen cells after the enrofloxacin treatment. Enrofloxacin inhibited the metabolic activity and mitochondrial membrane potential of LPS-treated spleen cells significantly. On the other hand, enrofloxacin did not alter the proportion of the subsets in spleen cells, and did not induce cell death. The production of tumor necrosis factor-alpha in LPS-treated spleen cells was inhibited by enrofloxacin. Overall, enrofloxacin had modulatory activity in spleen cells treated with LPS. These data may broaden the use of enrofloxacin as an antibiotic with anti-inflammatory activity in veterinary clinics.

Keyword

enrofloxacin; spleen cells; anti-inflammatory activity; lipopolysaccharide; modulatory action

MeSH Terms

Animals
Cell Death
DNA, Bacterial
Hand
Hospitals, Animal
Membrane Potential, Mitochondrial
Mice*
Spleen*
Tumor Necrosis Factor-alpha
Veterinary Medicine
DNA, Bacterial
Tumor Necrosis Factor-alpha