Exp Mol Med.  1998 Dec;30(4):199-204.

Induction of proliferation in resting B-cells by a factor released by activated mouse spleen cells

• Puri N
• Saxena RK

Abstract

Mouse spleen cells activated in a mixed lymphocyte reaction release a soluble factor, which induces a significant proliferative response in fresh mouse spleen cells. This proliferation inducing factor (PIF) was found to be heat stable (90 degrees C for 45 min) and also resistant to trypsin or chymotrypsin treatment. By using a sizing HPLC column, the molecular weight of PIF appears to be 25 kDa. Mouse spleen cells treated with anti-thy-1 + complement lost Con-A induced proliferative responses but responded well to PIF. B cell depleted spleen cells obtained by negative selection panning, did not respond to PIF. These results indicate that B cells proliferated in response to PIF. Polymixin-B, which blocks the B cell proliferative response to LPS, did not inhibit PIF induced proliferation.

Keyword

B cells; proliferation; cytokines; LPS; growth factor

MeSH Terms

Animal
B-Lymphocytes/physiology*
B-Lymphocytes/drug effects
Bone Marrow/metabolism
Cell Division/physiology
Chromatography, High Pressure Liquid
Chymotrypsin/pharmacology
Dose-Response Relationship, Drug
Growth Substances/pharmacology*
Growth Substances/chemistry
Heat
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Molecular Weight
Polymyxin B/pharmacology
Protein Denaturation
Spleen/metabolism*
Thymus Gland/metabolism
Trypsin/pharmacology