J Korean Fract Soc.  2003 Jul;16(3):423-431. 10.12671/jksf.2003.16.3.423.

Effect of Growth Hormone on Osteoblast and New Bone Formation

• Song HR
• Kang YJ
• Chang KC
• Yeon SC
• Koo JM
• Kim HH

• KMID: 1999369
• DOI: http://doi.org/10.12671/jksf.2003.16.3.423

Abstract

PURPOSE: To evaluate effect of growth hormone on osteoblast and new bone formation.
MATERIALS AND METHODS
Bone defect of the tibia with preserved periosteum was made and fixed with external fixator. Intramuscular injection of growth hormone for 8 weeks in experimental group and saline in control group was performed. New bone formation at the bone defect in radiograph and bone mineral density (BMD) by quantitative computed tomography were evaluated at 8 weeks after surgery. Rat osteosarcom cells were cultured in both group to evaluate cell viability of osteoblast, alkaline phosphatase activity, and mRNA expression of osteocalcin by RT-PCR.
RESULTS
Experimental group showed more callus formation and higher BMD at the bone defect site and the distal tibia compared to control group and there was significant difference. Proliferation of osteoblast, alkaline phosphatase activity, mRNA of osteocalcin at 5 days after culture were significantly higher in experimental group than those in control group.
CONCLUSIONS
Growth hormone has positive effect on osteoblast and callus formation in vivo and vitro studies.

Keyword

Growth hormone; Bone defect; New bone formation; Osteoblast

MeSH Terms

Alkaline Phosphatase
Animals
Bone Density
Bony Callus
Cell Survival
External Fixators
Growth Hormone*
Injections, Intramuscular
Osteoblasts*
Osteocalcin
Osteogenesis*
Periosteum
Rats
RNA, Messenger
Tibia
Alkaline Phosphatase
Growth Hormone
Osteocalcin
RNA, Messenger