Korean J Pediatr Hematol Oncol.  2000 Apr;7(1):57-63.

Correlation between Gene Expression of MDR1, Cyclin B, MAD2 and BAX in Childhood Acute Lymphocytic Leukemia

• Kim WD
• Kwon TK
• Choi KH
• Hah JO

Abstract

PURPOSE: Although outcome of the children with ALL has been improved remarkably with the multidrug chemotherapy and supportive therapy, relapse is still important cause of treatment failure. One of the mechanism related to the relapse has been reported to be multidurg resistance (MDR). To investigate the relation between the expression of MDR related MDR1 gene and cell cycle and apoptosis related genes in children with ALL, this study was conducted.
METHODS
The samples were collected from 9 children with ALL (5: at presentation, 4: at relapse) diagnosed at the pediatric department of Yeungnam University Hospital. From the mononuclear cells isolated from the peripheral blood or bone marrow, mRNA was extracted and analysed by RT-PCR. Using actin as a control, relative levels of mRNA of MDR1 gene, cell cycle control protein cyclin B and MAD2 and apoptosis related BAX gene were analysed.
RESULTS
The expression of MDR1 gene at the presentation and the relapse were variable and showed high correlation (Pearson correlation: 0.826) with the expression of BAX gene but low correlation with the expression of cyclin B and MAD2 gene.
CONCLUSION
These results suggest that mechanism involved in relapse of ALL include mechanisms other than MDR1 gene. High correlation between the expression of MDR1 gene and BAX gene suggests that high level of BAX expression increases probability of relapse but small sample size of this study precludes definite conclusion and further study is needed.

Keyword

MDR1; Cyclin B; MAD2; BAX; ALL

MeSH Terms

Actins
Apoptosis
Bone Marrow
Cell Cycle
Cell Cycle Checkpoints
Child
Cyclin B*
Cyclins*
Drug Therapy
Gene Expression*
Humans
Precursor Cell Lymphoblastic Leukemia-Lymphoma*
Recurrence
RNA, Messenger
Sample Size
Treatment Failure
Actins
Cyclin B
Cyclins
RNA, Messenger