Ewha Med J.  2006 Mar;29(1):3-9. 10.12771/emj.2006.29.1.3.

Role of Reactive Oxygen Species on Sodium Butyrate Induced Human Hepatocyte Differentiation

• Kim TH

• KMID: 1963995
• DOI: http://doi.org/10.12771/emj.2006.29.1.3

Abstract

BACKGROUND
/AIM: Reactive oxygen species (ROS) such as hydrogen peroxide, superoxide anion and hydroky radicals are produced in various physiologic and pathologic conditions and involved in many cellular processes as proliferation, differentiation and apoptosis. Studies investigating the role of ROS in various cellular behaviors especially in proliferation and apoptosis have been widely conducted in many cell types but the role of ROS in nontransformed human hepatocyte differentiation has not been investigated yet. thus we were going to elucidate the roleof ROS on human hepatocyte differentiaiton using sodium butyrate (SB) induced hepatocyte differentiation model of our own establishment.
METHODS
Intracellular ROS and apoptotic cell death were monitored by flowcytometry using peroxide sensitive probe (Dicholorofluorscein diacetate) and Annexin V/Propidium iodide, respectively. Urea nitrogen in culture media was measured by colorimetric methods. Ornithine transcarbamylase(OTC) and albumin trasncription was evaluated by RT-PCR.
RESULTS
Intracellular ROS production was increased by SB. SB induced urea production was significantly decreased with antioxidant treatment (p<0.05) and SB induced OTC and albumin transcription were also attenuated with antioxidant treatment. SB induced increase in apoptosiswas significantly inhibited by antioxidant treatment (p<0.05).
CONCLUSION
ROS produced during the process of sodium butyrate induced human hepatocyte differentiation auguments hepatoctye differentiation and apoptosis.

Keyword

Reactive oxygen species; Sodium butyrate; Human hepatocyte; Differentiation; Apoptosis

MeSH Terms

Apoptosis
Butyric Acid*
Cell Death
Culture Media
Hepatocytes*
Humans
Hydrogen Peroxide
Nitrogen
Ornithine
Reactive Oxygen Species*
Superoxides
Urea
Butyric Acid
Culture Media
Hydrogen Peroxide
Nitrogen
Ornithine
Reactive Oxygen Species
Superoxides
Urea