J Korean Assoc Maxillofac Plast Reconstr Surg.  2002 Jan;24(1):16-30.

The Effect Of Tetracycline On TNF-alpha And Lipid Peroxidation In Sepsis Model Of Rat

• Oh DJ
• Kim OH

Abstract

SIRS (systemic inflammatory response syndrome)is an unresolved problem,in which systemic cellular injury is induced by the inflammatory cells. In SIRS, TNF-alpha plays a key role as a mediator of inflammation and toxic oxygen radicals are known to be final effectors of stimulated leukocytes. Tetracycline (TC)is a broad-spectrum antibiotics which is effective to periodontitis and oral infections. There have been several reports on the non-antimicrobial effects of TC, such as the antioxidant effect and the TNF-alpha suppression effect. But the in vivo effect of TC on TNF-alpha production and peroxidation damage has not yet been elucidated exactly. This experiment is designed to evaluate the effects of TC on TNF-alpha production and lipid peroxidation in sepsis. Female Sprague-Dawley rats were divided into three groups : a control group, a group injected with TC, and a group injected with gentamicin (GM).Sepsis model was made by making large subcutaneous abscess in the back skin of the rats.After sacrificing five rats of each group at intervals of 24 hours and 48 hours after abscess formation respectively,the biochemical assays of malondialdehyde (MDA)levels,superoxide dismutase (SOD)activity and catalase activity in liver and lung tissue and TNF-alpha level and nitrate/nitrite level in serum were performed,and histological findings of liver and lung tissue were examined microscopically. Conclusively TC has a definite protective effect against lipid peroxidation and antioxidant enzyme exhaustion in sepsis, and has a tendency to suppress the nitric oxide secretion in sepsis.Unexpectedly TC has a tendency to augment TNF-alpha production in sepsis,which suggests no direct relation between TNF-alpha and oxygen radical production or nitric oxide production. Further evaluation is necessary to explain the mechanism.

Keyword

Sepsis; Tetracycline; Antioxidant effect; TNF-alpha; Nitric oxide

MeSH Terms

Abscess
Animals
Anti-Bacterial Agents
Antioxidants
Catalase
Female
Gentamicins
Humans
Inflammation
Leukocytes
Lipid Peroxidation*
Liver
Lung
Malondialdehyde
Nitric Oxide
Oxygen
Periodontitis
Rats*
Rats, Sprague-Dawley
Reactive Oxygen Species
Sepsis*
Skin
Tetracycline*
Tumor Necrosis Factor-alpha*
Anti-Bacterial Agents
Antioxidants
Catalase
Gentamicins
Malondialdehyde
Nitric Oxide
Oxygen
Reactive Oxygen Species
Tetracycline
Tumor Necrosis Factor-alpha